Antiseptic disinfectants

and sterilizations
GROUP 6
FACTORS IN SELECTING METHOD

 Nature of Organisms Present

 Number of Organisms Present
 Type of Equipment
 Intended Use of Equipment
 Available Means for Sterilization and Disinfection
 Time
PROCEDURES IN CLEANING
SUPPLIES AND EQUIPMENT
 Wear waterproof gloves if the articles are contaminated with organic material such
as feces, blood, pus, mucous, and if you have skin abrasions on the hands
 Rinse the articles first with cold running water to remove organic material.
 After rinsing them, wash the articles in warm water containing detergent or soap.
 Use a brush with stiff bristles to clean the articles thoroughly
 Rinse and dry the article thoroughly
 Prepare the cleaned equipment for sterilization or disinfection
 Consider the brush, gloves ,and the sink/basin that were used to clean the articles as
highly contaminated, and treat or dispose them immediately
PHYSICAL MEANS OF
STERILIZATION AND DISINFECTION
 Steam- Steam or moist heat is the most dependable and practical
means for the destruction of microbial life because it coagulates
proteins within the cells.
 Boiling Water- Inexpensive and simple, which makes it commonly
used in homes. Boiling an item in water for 15 minutes can achieve
disinfection.
 Dry Heat- Dry heat kills organisms by an oxidation process.
Disinfection occurs when a temperature of 160° C is maintained for 2
hours.
 Radiation- Ionizing radiation causes the death of microorganisms by
altering their essential metabolic processes.
CHEMICAL MEANS OF
STERILIZATION AND DISINFECTION
 Ethylene Oxide Gas- It is a chemical sometimes used for
sterilization. The gas destroys microorganisms by interfering with
metabolic processes in cells.
 Chemical Solutions- Generally used for instrument and equipment
disinfection and for housekeeping disinfection. Chlorine
compounds, iodine, and alcohol are the major classes of chemical
solutions.