Anti-osteoporotic Activity of Hydroalcoholic extract of

Lycopersicon esculentum Leaves in Chemicals Induced Osteoporosis In Female Wistar

T319
Rats
ABSTRACT RESULT
Effect of Ethanolic of Lycopersicon esculentum leaves in HISTOPATHOLOGICAL STUDY OF FEMUR BONE
Osteoporosis is the skeletal disease characterized by bone mass, Glucocorticoid induced Osteoporotic(GIO) Rats
BIO CHEMICAL PARAMETERS
deterioration of bone tissue experienced by women & elderly PHYSICAL PARAMETERS Serum Analysis Urine Analysis

individuals. Glucocorticoid and Heparin are used as positive Serum

Femur bone Femur bone Femur bone Alkaline Serum serum Urine
control to produce osteoporosis. Includes Physical & Weight volume density phosphatase Phosphorous calcium Urine calcium Phosphorous

Biochemical parameters. Rats were divided into 9 groups of 6 Normal Group 0.69 ± 0.12 0.48 ± 0.21 1.32 ± 0.32 168.5 ±2.74 4.67 ± 0.63 16.95 ± 1.32 0.61 ± 0.08 5.91 ± 0.44 Normal Control Standard Low Dose High Dose
animals. Doses of extract of Lycopersicon esculentum were 200 Control Group
Methylprednisolone 0.47 ± 0.24 0.38 ± 0.04 0.92 ± 0.16 351.2 ± 3.02 3.75 ± 1.26 13.06 ± 0.86 0.65 ± 0.07 5.96 ± 0.43
Fig: Heparin Induced Osteoporosis model in rats

and 400mg/kg. Ethanolic extract of Lycopersicon esculentum Standard group

Methylprednisolone
possess good Anti-Osteoporotic activity & antioxidant activity. +
Alendronate 0.64 ± 0.27a 0.45 + 0.06a 1.17 ± 0.29a 216.2 ±3.13a 4.28 ± 0.49a 15.9 ± 0.51a 0.55 ± 0.66a 5.88 + 0.57 a
Low dose group

INTRODUCTION Methyl prednisolone

+ ELE ( 200
Normal Control Standard Low Dose High Dose
Osteoporosis is a Systemic skeletal disease characterized by low mg/kg ) 0.52± 0.30b 0.36 ± 0.06b 0.99 ± 0.19b 249.3± 4.52b 3.43 ± 0.44b 13.6 ± 0.89b 0.41 ± 0.08b 5.83 ± 0.46b
Fig: Glucocorticoid induced Osteoporosis model in rats
bone mass & micro architectural deterioration of bone tissue with High dose group
Methyl
a consequent increase in the bone fragility and susceptibility to prednisolone + ELE CONCLUSION:
(400 mg / kg) 0.63 ± 0.34c 0.39 ± 0.05 c
1.08 ± 0.20 c
226.6 ±2.58 c
4.02 ± 0.52 c
14.9 0.38± 0.05 c
5.77 ± 0.31 c

fracture. It constitutes major health problem, leading to decrease Ethanolic extract of Lycopersicon esculentum possess good
Statistical significance of the results was tested by comparing
quality of life, disability & even death. Anti-osteoporotic & dose dependent increase in anti oxidant
treatment groups with the respective control group by employing
activity in DPPH radical scavenging activity.
one-way ANOVA and Dunnett's test where a , b , c p < 0.05 .
OBJECTIVES DISCUSSION
Physical parameters Chemical Parameters Alkaline Phosphatase
Osteoporosis is found that 50% women and 36% men over
The Objective of research work is to evaluate of
50years of age were noted to have low bone mass. GIO
Anti- osteoporotic activity of ethanolic extract of
used to treat inflammatory, allergic disorders, occurs due to
Lycopersicon esculentum in rats.
loss of bone density. Heparin is anticoagulant is used for
METHODS prophylaxis & treatment of thromboembolic disorders.
 Female Wistar albino rats (150 to 220g) were divided into 9 Lycopersicon esculentum is reported to possess
groups of 6 animals . polyphenolic compounds, vitamins and minerals reported
Effect of Ethanolic extract of Lycopersicon esculentum to have antioxidant & Anti-osteoporotic activity.
 Rats were treated with Lycopersicon esculentum leaves leaves(ELE) in Heparin induced Osteoporosis(HIO)rats
extract. REFERENCE:
PHYSICAL PARAMETERS CHEMICAL PARAMETERS
Serum Alkaline Urine 1. Gutierrez RP. Review of Lycopersicon esculentum its
Femur parameters: Bone weight, volume, density. Normal Group
Femoral weight
0.70 ± 0.09
Femoral volume
0.48 ± 0.21
Phosphatase
168.5 ± 2.74
Phosphorous
5.91 ± 0.44
phytochemistry and pharmacology. Med. Chem.
 Serum Parameters: Alkaline Phosphate, Calcium, Control group 2016;6:12-21.
Heparin 0.54± 0.03 0.32 ± 0.05 303.7 ± 1.87 5.15 ± 0.58
2. JAMA N. Consensus Development Panel on Osteoporosis
Phosphorous. standard group
 Urine Parameters: Calcium, Phosphorous.
Heparin Prevention, Diagnosis, and Therapy. Osteoporosis
+Alendronate 0.65 ± 0.06a 0.41 ± 0.05a 167.9 ± 1.90a 5.54 ± 0.41a
 Histopathological Studies. Low dose group Heparin +
prevention, diagnosis, and therapy. J Am Med Assoc.
 Antioxidant activity
ELE(200mg/kg) 0.54 ± 0.02b 0.34 ± 0.04b 255.5± 1.42b 5.06 ± 0.19b 2001:85:785-95.
High dose
Heparin + ELE ( 400 mg 3. Murali Krishna et al., [Phytochemical Analysis and
/kg) 0.61 ± 0.06c 0.38 ± 0.06c 175.9 ± 1.73c 4.97 ± 0.55c
 The induction assessment of Glucocorticoid and Heparin IN VITRO ANTIOXIDANT % Inhibition of % Inhibition of Antimicrobial Studies of Various Extracts of Tomato
Concen Ascorbic Lycopersicon
ACTIVITY (Solanum Lycopersicon L.)], Sch. Acad. J.Biosci., 2013;
induced osteoporotic effect of the extract was studied through tration acid esculentum

Lycopersicon esculentum shows 5 38.51 14.36 1(2):34-38

Physical & Biochemical Parameters. 10 61.05 34.41
 At the end of the study rats were sacrificed & DPPH free radical scavenging 20 73.81 39.73
4. Osteoporosis NI. Related Bone Diseases National
activity with IC50 value 30μg/ml 40 81.92 59.78 Resource Center. The Surgeon General's report on bone
Histopathological studies of femur bone was done.
 Data were expressed as ±S.E.M and the results were compared to std ascorbic acid which 60 90.53 69.80 health and osteoporosis: what it means to you. Accessed
80 91.08 84.28
was 8μg/ml. June 6, 2017). Available at: https://www.bones. nih.
analyzed by ANOVA & Dunnett's test. 100 93.37 93.55
IC50 ~ 8 μg / ml ~ 30 μg / ml gov/health-info/bone/SGR/surgeon- generals-report. 2012

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