Evaluation of protective effects of Saraca asoca (Roxb.)Willd. and Mimusops elengi L.

fruits

on neurobehavioral activity against stress-induced rodents

Presented in partial fulfillment for the award of the degree of

Doctorate of Philosophy in Pharmacognosy

By
Mr. Ali Husen
(Enrollment number - 2001101)
Faculty of Pharmacy

Supervisor Co-supervisor
Dr. Muhammad Arif Dr. Aleza Rizvi
Associate Professor (Jr.) Prof. & Head
D/o Pharmacognosy, F/o Pharmacy, Hygia Institute of Pharmacy,
Integral University, Lucknow
Lucknow-26, India
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Aim and Objective

1. Pharmacognostical study of Saraca asoca (Roxb.) Willd and Mimusops elengi L. fruits.

Morphological study
Microscopical study
Estimation of foreign matters
Loss on drying
Ash value
Extractive value
Fluorescence analysis of fruit powder
Extractions of crude drugs
Phytochemical screening
Thin Layer chromatography
2. Extraction, Fractionation and Characterization of fraction by Chromatograhic
techniques (GC.MS).

3. Evaluation of anti-stress effects of fractions

-Evaluation of neurobehaviour parameters-

Effect on motor coordination using rota rod

Effect on behaviour using elevated plus maze

-Evaluation of Immunomodulatory parameters

Footpad reaction test

Heamoglutination titre

4. Evaluation of Anti-oxidant activity

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 Objectives covered in past 6 months

Macroscopic Characteristics

Mature fruits are dark red while young fruits are light green in colour.

The ovoid to oblong fruit is up to 35mm long and 16mm wide

They are taste like dates but to be more dry and deed are dark brown in color

Fig. 1 Morphological view of Mimisops elengi fruit

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Foreign matter analysis

Wt. of crude drug (gm) Wt. of crude after Wt. of foreign matter foreign matter (%w/w)
removal of foreign (gm)
matter (gm)

10 9.92 0.08 0.08

10 9.93 0.07 0.07

10 9.93 0.07 0.07

Mean - - 0.73

Table. 1

Loss on drying
Wt. of China dish + Wt. of China dish + A-B (gm) Loss on Drying
drug (before drying) drug (after drying) (%w/w)
(gm) A (gm) B

52.46+10=62.46 60.60 1.86 18.6

60.34 2.12 20.2
60.34 2.12 20.2
Mean - - 19.66

Table. 2
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 Fluorescence analysis of fruit powder

Solvents Used Observation

Day Light UV 254nm UV366nm

Drug p/w as such Yellow Light Green Dark Green
Ethyl Acetate Yellow Green Greenish Black
Distilled Water Yellowish Brown Green Greenish Black
HCl Dark Brown Greenish Black Black
Picric Acid Light Green Dark Green Black
Acetone Dark Yellow Dark Green Greenish Black
NaOH in Methanol Yellowish Brown Green Black
Ethanol Yellowish Brown Light Green Dark Black
Toluene Yellowish Brown Green Black
Conc. Sulfuric acid Dark Brown Black Black
Nitrobenzene Green Black Black
Ferric chloride Green Black Black
Methanol Yellowish Brown Green Greenish Black

Table. 3
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 Extractive value

Cold percolation
Solvent Wt. of drug (gm) Color extract Extractive value
matter (%) Mean
Pet. ether 5 Pale yellow 1.4

Chloroform 5 Yellowish orange 2.2

Ethanol 5 Rusty orange 11.9

Aqueous 5 creamish 9.3

Table. 4

Fig. 2 Pet. ether, Chloroform, Ethanol, Aqueous extract of Mimusops elengi

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 Hot extractive

Solvent Wt. of drug (gm) Color extract matter Extractive value (%)
Mean
Pet. ether 20 Orange 1.77

Chloroform 20 Yellow 2.27

Ethanol 20 Brown 14.25

Aqueous 20 Dark brown 12.5

Table. 5

Fig. 3 Pet. ether, Chloroform, Ethanol, Aqueous extract of Mimusops elengi

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Successive extraction

Solvent Wt. of drug (gm) Color extract Extractive value

matter (%) Mean
Pet. ether 20 Orange 1.27

Chloroform 20 Creamish 1.4

Ethanol 20 Light creamish 12.07
Aqueous 20 Light brown 8.97

Table. 6

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Future work plan
• Extractions of crude drugs
• Phytochemical screening
• Thin Layer chromatography
• Extraction, Fractionation and Characterization of fraction by Chromatograhic
techniques(GC.MS).

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THANK YOU

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