IONIZATION EQUILIBRIUM OF GLYCYLGLYCINE

Laboratory of Physical Chemistry

Working Group: PL2_G6_03 Luís Manuel Belchior and Carlos Filipe Lima

AIM /Scope
PHYSCHEMLab #03

The objective of this work is to study the ionization balance of diprotic acid (glycylglycine) using potentiometry and conductimetry.

GGH+: GG: Glycylglycine GG-: Glycylglycinate

2-(carboxymethyl)-2-oxoethan-1-amine

Ka1:

Ka2:

Methodologies & Application

• Firstly, three NaOH titration tests were carried out.
• After, the titration of Glycylglycyl Chloride was carried out. As the titrant
(NaOH) was added, the pH and electrical conductivity were recorded.

Concentration Concentration of Volume of

of HCl/M Glycylglycine/M Glycylglycine/cm3
|𝑁𝑎𝑂𝐻|=𝑉 𝐻𝐶𝑙∗∨𝐻𝐶𝑙∨ ¿ ¿
0,1010 0,03 50 𝑉 𝑁𝑎𝑂𝐻

Table 1 – Values of concentration and volumes utilized.

Figure 1 – Experimental setup for NaOH titration (right), glycylglycil
chloride titration (center) and pHmeter and conductivimeter (left).

Results & Achievements

Concentration of
0,1036
NaOH/M

Volume NaOH/ cm3

Vensaio 1 14,68
Vensaio 2 14,60
Vensaio 3 14,59
Table 3 – Experimental results for the main titration.
Table 2 – Values of NaOH titration.

• With the NaOH titration, it was possible to conclude that the concentration of
NaOH solution was 0,1036 M. Figure 2 – pH and K versus the volume of NaOH.
• Analyzing the pH and K versus the volume of NaOH, the titration curve is lined
with expectations for a titration of a weak diprotic acid with a strong base, 1st derivative
2and Derivative
1º & 2and derivative = f (V_NaOH)
obtaining two equivalent points. The conductimetry curve also carried the 5.000 3.000

expectations according with the specimens in each step of the titration. 4.500
2.000

• The titration curve was derived twice in order to obtain the two equivalent 4.000

1.000
volumes with more precision. Following the data treatment, it was possible to 3.500

3.000
obtain the acidity constants and the concentration of the sample.
2and Der

0.000
1St Der

2.500
• The values for the acidity constants are close with the searched literature. -1.000
2.000
Gridchin (2016) obtained values of pKa1 and pKa2 of 3,16 and 8,31,
1.500 -2.000

respectively, for Glycyl-glycine samples. 1.000

-3.000
0.500

0.000 -4.000
0.0000 5.0000 10.0000 15.0000 20.0000 25.0000 30.0000 35.0000 40.0000

V_NaOH / cm3

References: Figure 3 – First and Second derivate versus the volume of NaOH.

GRIDCHIN, S. N. Thermodynamic Characteristics of Protolytic Equilibria in Aqueous Solutions of Glycyl

Peptides. Russian Journal of Physical Chemistry A, 2016, Vol. 90, No. 11, pp. 2170–2176.
VBADELINA, V. G.; BARANNIKOVA, V. P.; TARASOVAA, G. N.; CHERNYAVSKAYAB, N. V.; KATROVTSEVAB, A.
V.; FAM TKHI LAN. Thermodynamical Characteristics of AcidBase Equilibria in Glycylglycine Aqueous
Solutions at 298 K. Russian Journal of Physical Chemistry A, 2012, Vol. 86, No. 1, pp. 40–44

LabQF2024
EXP.03 Laboratório de Química-Física