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*Study of antibacterial properties of

some Nano compounds against
clinically isolated bacterial

‫دراسة الخواص المضادة للبكتريا لبعض‬

‫مركبات النانوعلى البكتريا المعزولة سريريا‬
 Presented by

*Chem/ Eman Hussein Mahmoud

( 2020 )
 Supervised by

Prof. Dr. Osama Mohamed Abdel Raouf

Prof. of Bacteriology, Department of Botany and Microbiology ,Faculty of
Science , Aswan University
Dr. Nora Sharkawy Abazid
Assistant Prof. of Bacteriology, Department of Botany and
Microbiology ,Faculty of Science , Aswan University

Dr. Hisham Abdel Moneim Abo Elmagd

Lecturer of Bacteriology, Department of Botany and Microbiology ,Faculty of
Science at Al-Azhar university Assiut
 *Introduction
Antibiotic Resistance:
 occurs when bacteria change so
that antibiotic medicines can’t kill them or stop
their growth. As a result, bacterial infections
become extremely difficult to treat.
 various strains of bacteria have adapted to the
medicines that typically kill them. They can fight
back against the drugs. Called superbugs, these
bacteria continue multiplying and causing infections
despite treatment with several different antibiotics.
There’s a chance that no antibiotic will work.
*complications of antibiotic resistance:
*Increased risk of severe, extended illness and death.
*Severe medication side effects.
*Longer hospital stays.
*More medical appointments.
*Increased medical costs
Who is at risk for harm from antibiotic resistance?
Older people and those with weakened immune systems
Have AIDS, Cancer, Are an organ transplant stem cell (bone
marrow) transplant recipient.
Pathogenic bacteria
 Pathogenic bacteria are bacteria that
can cause disease and damage cells and
tissue by releasing toxins.
 Not all bacteria are pathogenic, in the
body , there are many types of harmless
bacteria ,and some may even support
essential bodily functions.
 Doctors typically prescribe antibiotics to
treat bacteria infection, but some are
becoming resistant to these drugs.
Some examples of pathogenic bacteria :
*Pseudomonas aeruginosa - can cause pneumonia and blood
infections.
*Mycobacterium tuberculosis - causes tuberculosis.
*Listeria - causes foodborne infections and flu-like
symptoms.
*Salmonella - causes typhoid.
*E.coli - causes intestinal infections with symptoms like
fever and diarrhea.
* Staphylococcus saprophyticus /Streptococcus pyogenes
* Proteus sp./Klebsiella granulomatis /Neisseria
gonorrhoeae /Staphylococcus aureus / Chlamydia .
Nanoparticles :
 spherical, polymeric particles composed of
natural or artificial polymers.
 NPs are tiny materials having size ranges
from 1 to 100 nm.
 The different groups
include fullerenes, metal NPs, ceramic
NPs, and polymeric NPs.
 be classified as hard (e.g., titania [titanium
dioxide], silica [silica dioxide] particles,
and fullerenes) or as soft (e.g., liposomes,
vesicles, and nanodroplets
Ai
m
 *Aim of the work

1-isolation and identification of the most

potent bacteria in pneumonia

2-Studying the antibacterial properties of

some Nano compound on most potent
isolated.
 Materials and methods
1) Preparation of different type of media as
( Maconky – MSA – blood agar – Nutrient Agar )

2 ) 50 sputum samples were taken from patients in

different parts of the body.

3 ) We plant each sample on the 5 different types of

media so we get 250 petri dishes with different
types of pathogenic bacteria .
4 ) Make isolation of the bacteria formed on petri

Then Identification of samples by :

( selective media – Biochemical test )

5 ) By Data analysis select the most potent of

organisms.

6 ) Antibacterial test by different Nanoparticles

compound ( MIC / MBC )
 MIC : is Minimum inhibitors concentration.

MBC : is Minimum bactericidal

concentration
‫تم بحمد هللا‬