Introduction to Laboratory

30.03.2023
Çağla Ece Olgun
B57, colgun@metu.edu.tr
Pipettes

Pipette tips
Pipettes

Electronic pipette pomps

Serological pipettes

Manual pipette pomps

Pipettes
Laboratory equipments
Graduated cylinder:
• To measure the volumes correctly.
• Also called as measuring cylinder.
Erlenmayer flask
• Used in microbiology to grow organism.
• It is good for the aeration of the
microorganisms.
• Used to heat and store solutions
• Also called as measuring cylinder.
Beaker
• A volumetric flask with a wide range of usage
area.
• Used to stir, heat (if glass), measure the liquid
volume and mix the reagents
Laboratory equipments
Petri Dishes
• Also called as petri plate or cell-culture
dish.
• It is used in biology widely to grow
different organisms or cells such as
plants, mammalian cells in cell culture or
bacteria/yeast.
Microtubes
• single-use tubes made from polypropylene
for preparing, mixing, centrifuging,
transporting and storing solid and liquid
samples and reagents.
• Also called as eppendorf tubes.
Falcons
• Polypropylene tubes that can be 15 or 50 mls.
• Preperation of buffers, solution storage,
centrifugation, etc.
Laboratory equipments

Centrifuge Bunsen Burner Autoclave

• An apparatus that rotates at high speed
and separates substances of different
densities.
• It should be used in balance; otherwise,
it might explode.
• A common piece of laboratory equipment
that produces single open gas flame, which is
used for sterilization and heating.
• Important for aseptic techniques.
• A pressure chamber to sterilize equipment
and supplies by subjecting them to high
pressure saturated steam at 121oC for
around 15-20 min depending on the size of
the load and the contents.
• It is used to sterilize culture media, discarded
biological waste and other equipments.
Aseptic Techniques
• Aseptic techniques are the precautionary measures taken in order to
prevent contamination of pure cultures and sterile laboratory
equipment.
• The term “aseptic” literally means “without contamination.” These
procedures are as important for the experimenter’s safety as they are for
maintaining culture purity.
• Sterile: Devoid of all life forms (bacteria, fungi,viruses)
• This is all or none.
• Aseptic techniques includes:
• cleaning the bench top work areas with disinfectant solution,
• washing hands with alcohol before starting work ,
• work near the flame,
• use sterile media and glassware.