Optical Trapping and

Tweezers
 Table of contents
 Basic introduction
 Basic Phenomenon
 Regimes
 Applications of OTs on
single molecule level
 Instrumentation

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 Hi!
I’m Syeda Laiba Shah

I’m here to present:

 Optical Tweezers
 Radiation pressure
 Phenomenon
 Ray optic Regime

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 • Technique for
Optical microscopic trapping
Trapping • Manipulation of
particles

• Instruments used in
Optical optical trapping
Tweezers • By Arthur Ashkin in
1985

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 Radiation Pressure
“Optical trapping basically uses
radiation pressure to trap particles.”

Photon P==

Forces:
 Single photon momentum = kgm/sec
 Laser(mW) = photons/sec
 Force = Newtons
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 Detailed View
 Proper explaination of the process depends on particle size.
 Two regimes used are:
i. Ray optics regime
ii. Rayleigh regime
Ray
optics • a˃λ
Rayleigh • a<λ
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 Ray optics Regime:
Force of refraction Force of reflection
pushes bead toward pushes the bead
focus. forward.

Case 1

Lateral opposite force Trapping of bead at

in action. focus.

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 Conti…..

Case 2

Bead
Restoring Bead pushed
displaced
force in action towards centre
from centre

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 Focused laser(bead Unfocused laser(bead is
displaced in center) displaced from center)

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 Manipulate and Measure:

 F = kx
 K = Spring constant
 X = Bead
displacement from
trap centre
 F = 6πɳrv

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 Hello!
I’m kainat Javed

I’m here to present:

 Applications of OTs at
single cell level
i. Microorganisms in trap
ii. Motion of Kinesins

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 Application of OTs at single cell Level:
 In trapping microorganism
 Confinement and organization of cells
 Tracking of movement
 Studying of nanobiological mechines
 Cell sorting
 Analysis
CREDITS: oftemplate
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 Study of RCBs
Tandri

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 Microorganisms in trap
 First microorganisms trapped
was paramecium
 50 to 330 micrometer in length
 Internal components detected
 Manipulation of all sorts of
cell

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 Picture of paramecium where one can see the internal
organelles. The arrow points to the specific organelle
that we manipulated using an optical tweezer.

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  Analysis of dynamics and
Motion of Kinesins properties
 Behaviour of motion studied
Observations:
 Kinesins attach to microtubules
 Displacement covered was
measured
 Each step is 8nm

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 (a) A schematic representation of the transport of a transparent
bead by a kinesin molecule walking on a microtubule.
(b) Three measurements of the position of the bead with time
showing that the motion occurs by discrete steps of 8nm.

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 Hi!
I’m Khansa

The topic of my presentation is:

 Applications
i. RBCs in trap
ii. Gene transcription

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 RBCs in Trap  Measurement of cell
deformability
 Cell stiffness was observed
 Interactions of RCBs with
parasites studied
 Study of movement behaviours

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 (a) Schematic representation of an RBC trapped in the middle of two
opposing laser 1 and 2, forming an optical stretcher.
(b) The principle of the optofluidic “tweeze-and-drag” cell stretcher.

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  Dual optical tweezers were used
Gene Transcription  Analysis of gene transcription
 Formation of dumbell assay
 Observations
 Forces and displacement measured
 RNAp movements measured
 Location changes observed
 Back tracking
 Transcriptional pausing

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 Two optical tweezers hold
transparent spheres with
one attached to a DNA
templete and the second
attached to an RNA
polymerase enzyme,
actively transcribing RNA.

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 Hi Friends!
My name is Ayesha Shabeer

I would like to present:

 One of the application that
is Cell sorting
 Instrumentation

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  Single cell isolation
Cell Sorting  Development of “Easy sort
technique”
 Particularly isolates yeast,
bacterial and human cells
 Doubling time of yeast cell
observed
 Diameter was observed

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 Yeast Cells

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 Laser

Beam
Optics
expender
Setups

Tweezer with
Microscope
imaging
objectives
techniques
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 Diagram

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