Department of Biotechnology ; Motilal Nehru National Institute of Technology Allahabad Prayagraj, 211004

Efficient Conversion of FastQC Data into Raw Count Table: A Linux-Based Procedure

Next-Generation Sequencing (NGS) is a set of

revolutionary genomics technologies that allows for
rapid and cost-effective sequencing of DNA or RNA.
Unlike traditional methods, NGS enables the parallel
sequencing of millions to billions of fragments,
facilitating the analysis of entire genomes,
transcriptomes, and epigenomes .
With the advancement in NGS technologies, the cost
of sequencing has reduced significantly over the
years, enabling researchers to opt for RNA-
sequencing instead of microarrays for transcriptome
analysis. After the isolation of mRNA from
adenocarcinoma cells; cDNA libraries were
constructed and was subjected to Illumina HiSeq
2500 to generate FastQC files. The fastqc files were
aligned to the human reference genome (GRCh38)