How to identify common

pathogens?
Dr.Vrushali Thakar
 Gram’s staining

S.aureus-GPC in clusters Streptococci- GPC in chains

Pneumococci- GPC in pairs

 Meningococci- GNC in pairs Gram negative bacilli

C.Diphtheriae in Albert’s stain C.diphtheriae in Gram’s stain

 Culture plates

Pseudomonas aeruginosa on NA Antibiotic sensitivity testing

Swarming growth of Proteus on BA LFF or NLF colonies on MacConkey's agar
Streptococcus pneumoniae on BA

Streptococcus pyogenes on BA
C. Diphtheriae on blood potassium tellurite agar
S.aureus on blood agar
 Anaerobes

Robertson’s cooked meat medium Gas Pack Jar

 Common anaerobes
• Clostridium welchii
• Clostridium tetani
• Clostridium botulinum

• Nonsporing anaerobes- Peptococci,

peptostreptococci, veillonella,
• prevotella sp, Bacterioides, Porphyromonas,
Fusobacterium etc
Mycobacterium Tuberculosis
Aspergillus niger
Mucor
Candida sp
Chrome agar for Candida sp identification