IN-VITRO ANTIBACTERIAL

ACTIVITY OF ‘TRINETRAKHYA
RASA’
ON MUTRAKRCCHA.
SUPERVISOR
VD. MANOJ KUMAR BHATI
M.D.(Ayu)
(ASSOCIATE PROFESSOR)
PRESENTED BY
RIDHI MEHTA
(M.D 1st YEAR)

DEPARTMENT OF RASA SHASTRA EVAM BHAISHAJYA KALPANA

PATANJALI BHARTIYA AYURVIGYAN EVAM ANUSANDHAN SANSTHAN, HARIDWAR-

249405
 RESEARCH QUESTION?

To assess the efficacy of Trinetrakhaya Rasa in the zone of

inhibition against E. Coli bacteria, where the formulation is
mentioned in Rasendra Sara Sangraha under Mutrakrccha
cikitsa ?
 INTRODUCTION:
India has a wide variety of culinary, medicine and health customs. Of all the traditional knowledge system,
Ayurvedic science which is an ancient science offers insights for living a long and healthy life.

Acharya Sursruta describes health using the phrase that explains the equilibrium state of Dosha, Agni, Dhatu,
Malkriya can be achieved by having good diet and exercise.

lenks"k% lekfXu'p le/kkrqeyfØ;%A

çlUukResfUæ;euk% LoLFk bR;fHk/kh;rsAA lq-lw-ƒ‡A†Š

Despite this, Acharya Charak explains (Trividha roga ayatana) that there are three causative factors that will lead to
disease.

vFkkZuka deZ.k% dkyL; pkfr;ksxk;ksxfeF;k;ksxk%A p​-lw-ƒƒA…‰

Rasa Shastra and Bhaishajya Kalpana hold the significant role within the field of ayurvedic treatment modalities and
pharmacy. The term Rasa Shastra refers to as the (Science of mercury, metals and minerals). Bhaishajya Kalpana means the
 TO BE CONTINUE..
The concept of three essential elements for the body’s formation and maintenance are Dosha Dhatu Mala Mulam hi
Shareeram whereas Trimala is Sveda(sweat), Mutra(urine), Purish(stool). Mutra is the liquid waste product which should be
excreted out from the body by the processing through mutravaha strotas. Mutravega is considered as Adharniya vega. Basti
is the strotomoola of the Mutra which is considered as one among Trimarma. Our Acharyas have expounded on various
disorders associated with Mutra, Mutrakrccha is one among them. They have been discussed extensively about Mutrakrccha
which explains the etiopathogenesis and symptoms as well as treatment of 8 different types of Mutrakrccha.

Dosha-Prakopa is the root cause for the manifestation of Vyadhi. Anuchit Ahaar(food) and Vihaar(lifestyle) are important
factors in provoking the imbalance of doshas which are Ativyayam, Teekshana or Ruksha Aushadha, Madhyapana,
Prshthayam, Anoopamaans , Matsyasevan, Adhyashan, Ajeerna. Etc.

Mutrakrccha indicates Krcchta in Mutravahana which is denoted as Dysuria (painful and burning micturition) which can be
caused by all the factors responsible for the retention of urine and infections like Urinary tract infection.
In Ayurveda, Krimi is associated with microbes which has been shown since the Vedic period,
therefore the concept is not new. Acharya’s were aware of several diseases brought on by Krimi, or
germs. In Harita Samhita among the types of Bahya Krimi, Mutraottpanna Vartula is mentioned.
So, it can be considered as a micro- organism for UTI indicating the Ayurvedic landmark of
bacterial of bacterial origin of mutrakrccha.

Numerous Ayurvedic scriptures have described the drugs used in the treatment of Mutrakrccha.
Trinetrakhya rasa is a Herbo-mineral formulation which classified under Khalviya Rasayana is
mentioned in Rasendra Sara Sangraha (Mutrakrccha cikitsa) is one of the prominent texts of
Rasa Shastra. It is a significant and valuable book which is written in 14 th century AD by Shri
Gopalkrishna Bhatta in Sanskrit.
 MODERN ASPECT :

• UTI is the most common contaminated bacterial infection which can affect any region of the specifically
lower urinary tract.
• The most common presentation is acute urethritis or cystitis and typically manifests as abrupt onset of
frequency of micturition and urgency, cloudy or unpleasant odour, suprapubic pain during and after voiding.
• The main causative factors that contribute to the mechanism of causing UTI is the incomplete emptying of
the bladder, lower urethra is colonised with bacteria, immunocompromising disorders, foreign bodies, pH or
concentration of urine.
• Most acute episodes of UTI are associated with a possibility of future recurrent attacks if the underlying
cause cannot be removed. It refers to the urothelium of susceptible person may have receptors to virulent
strains of E.coli adherent.
• The Enterobacteriaceae family of bacterial pathogens, which includes Escherichia coli (80%), is the major
source of UTI. Other pathogens followed by Staphylococcus saprophyticus (10-15%), Klebsiella,
Enterobacter and Proteus species, and enterococci infrequently cause uncomplicated cystitis and
pyelonephritis.
• Although yeast, fungi and viruses may produce infection.
 NEED OF THE STUDY:
• Urinary tract infection is the major global health concern because of the potential long-term
harm to one’s quality of life. According to survey, the prevalence among females are much more
prone to males mainly due to female lower urinary tract anatomy and its close proximity to the
area contaminated heavily with bacteria.

• The lacunae is that modern science has been using antibiotics for treating UTI have some
limitations due to their antimicrobial resistance.

• Therefore, in order to enhance quality of life and boost immunity, the management criteria
should be created in addition to treating the symptoms. An Ayurvedic scholar has a moral
obligation to study the treasure trove of medicinal knowledge found in our Samhita for the
perfect cure.

• This Trinetrakhya Rasa formulation has received relatively little prior research.
 PREVIOUS WORK:

• PHARMACEUTICAL STUDY OF TRINETRA RASA

(Arathy Krishnan 1*, K. Unnikrishna Pillai 2 , Vineeth P.K 3 1PG
Scholar, Department of Rasashastra and Bhaishajya Kalpana,
Amrita School of Ayurveda, Kollam, Kerala, India.)

• Pharmaceutical And Analytical Study Of Trinetrakhya Rasa .

( Rasa Shastra . Maharashtra University of Health Sciences,
Nashik . 2011.)
 AIM AND OBJECTIVES:

Literature evidence shows that the Trinetrakhya Rasa has such properties to cure all types of Urinary
tract infection which are cover under Mutrakrccha. Therefore, this topic In- vitro study of Trinetrakhya
rasa on Mutrakrccha has been selected for the study.

1. To prepare Trinetrakhya rasa

2. To assess the physio-chemical analysis of Trinetrakhya Rasa.

3. To conduct Antibacterial study against E. coli bacteria.

 REVIEW OF LITERATURE:

1. AYURVEDIC REVIEW:
• The review of literature includes proper screening of classical texts, in which some
classical text name this formulation as Trinetra Rasa where near about 10 compositions are
available under this name. However, they all are differ from each other in all aspects. This
particular composition is mentioned in Rasendra sara sangraha, Rasendra cintamani,
Dhanvantri, Rasrajsunder, Rasyogasagar.

• The method of preparation of Trinetrakhya Rasa is according to Rasendra Sara sangraha.

 MATERIAL AND METHOD:

2. Classical reference:
o³~x lwra xU/kda Hkkof;Rok ykSgs ik=s enZ;snsd?klze~ A

nwokaZ;"Vhxks{kqjS% 'kkYeyhfHk% ew"kke/;s Hkw?kjs ikpf;Rok AA

rÙkn~HkkoSHkkZof;Rok·L; oYya n|kr~ 'khra ik;la o{;ek.kE A

nwokZ;"Vh'kkYeyh rks;nqX/kSLrqY;S% dq;Z~;kr~ ik;la ríhr AA

çkr%dkys 'khrikuh;ikukr~ ew=s tkrs L;kr~ lq[kh p Øes.k AA

j​-lk-la- ew=—PN fpfdRlk (1-2)

 MATERIAL AND METHOD:
SOURCE OF DATA-
• LITERARY SOURCE- All the relevant classical and up to date literature, various articles
on ‘Trinetrakhya ras’ will be screened for Antibacterial properties and will be reviewed.
• PHARMACEUTICAL SOURCE- Genuine raw materials will be collected from authentic
sources. Preparation of ‘Trinetrakhya ras’ will be done in pharmacy section of PG
Department of Rasa Shastra Evam Bhaishajya Kalpana, Patanjali Bhartiya Ayurvigyan
Evam Anusandhan Sansthan, Haridwar.
• IN VITRO STUDY - The study for evaluation of antibacterial activity of ‘Trinetrakhya ras’ will be
done through any one of the following methods- agar diffusion, other diffusion method on e.coli
growth.
• ANALYTICAL METHOD-Physio-chemical analysis of Trinetrakhya Rasa includes- Organoleptic
characters colour,odour, consistency etc., pH, Particle size, Loss on drying, Hardness,Total Ash,
Acid- insoluble Ash, Water soluble Ash ,Test for heavy/ toxic metals etc.
All the relevant and contemporary analytical studies shall be done.
 DRUGS :

Mineral:
• Parad (Mercury) - 1Part
• Gandhak (Sulphur) - 1Part
• Vanga Bhasma (Tin) - 1Part
Liquid Media:
• Durva (Cynodon doctylon) - 1Part
• Yashtimadhu (Glycerrhiza glabra) - 1Part
• Gokshura (Tribulus terristrus) - 1 Part
• Salmali (Bombax malabaricum) - 1 Part
 MATERIAL AND METHOD:

3. Name of the procedure including :

 Shodhan of Parad will be done according to Rasa Tarangini 5/34-35.

 Shodhan of Gandhak will be done according to Rasa Tarangini 8/13-17.

 Samanya Shodhan of Vanga will be done according to Rasa Ratna Samuchaya 5/13.

 Vishesha Shodhan of vanga will be done according to Rasa Tarangini.18/11-12.

 Jarana of Vanga will be done according to Rasa Tarangini.18/19-24.

 Bhasma of Vanga will be prepared according to Rasa Tarangini.18/19-24.

 PREPARATION OF TRINETRAKHYA RASA :
o Preparing Kajjali- By doing Mardan(grounding) Gandhak(sulphur) to Parad(mercury) in a Khalva yantra
(iron mortar and Pestle) without adding any liquid media till it becomes a homogeneous mixture.

o Kajjali that had already been prepared will be add together with Vanga Bhasma and thoroughly triturated;
this process will repeat until it becomes a mixture.

o Bhavna (triturate/ levigate) to the mixture using the designated liquid medium specified for a day with each
separately. SI. NO. Drug Latin name Quantity

1. Durva Cynodon doctylon 1 part

2. Yashtimadhu Glycerrhiza glabra 1 part

3. Gokshura Tribulus terristrus 1 part

4. Salamali Bombax malabaricum 1 part

 TO BE CONTINUE..

o Prepare a bolus, place them in a Musha (crucible/ cupel) and process

it by following the Bhudhara yantra method.

o Gather the material for the Bhavana annealing (triturate/ levigate)

once more using the liquids mentioned above.

o The final step will be preparing a tablet with 1 vallal (3Ratti- 375mg)
Matra dried it in shade and store it in a tightly closed glass container.
 DISCUSSION AND CONCLUSION:

• The assessment of antibacterial activity will be

done according to microbiological parameters
and the result will be analyzed.
• Following a statistical analysis and logical
discussion, a conclusion will be reached based
on the data collected using different approaches.
THANKYOU..