Impact of Synthetic Fertilizer

(NPK) on Soil Microflora &

germination of Vigna radiate
cultured on Virgin Soil
PRESENTED BY: GUIDED BY:
Shakti P Mishra BIKRAM PRADHAN
Satyabrata Rath ASSISTANT PROFESSOR
Sipra Moharana
S.V.N. Sireesha
Simran Sahu
Amisha Pattanaik
Ayesha Biswal
DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BASIC SCIENCE & BIOTECHNLOGY
NIIS INSTITUTE OF INFORMATION SCIENCE & MANAGEMENT

1
 Impact of Synthetic Fertilizer (NPK) on Soil Microflora & germination of Vigna radiate cultured on Virgin Soil

INTRODUCTION OBJECTIVES
 The use of fertilizers contributes to  Collection of Virgin soil sample form the Buffer Zone of
global food security by ensuring Chandaka Forest reserve.
higher crop yields, which is crucial  Testing the Physical and Chemical Properties of soil
to meet the demands of a growing samples.
population.  Studying the effect of Synthetic Fertilizer (NPK) on Soil
 To minimize the negative Microbes when used in different concentration.
environmental impact of fertilizers,  Studying the effect of Synthetic Fertilizer (NPK) on
it is crucial to adopt sustainable growth and yield of Mung bean (Vigna radiate).
agricultural practices, including
precision farming, proper nutrient
management, and the use of
environmentally friendly fertilizers.
 Sustainable agriculture practices,
including the use of organic
fertilizers and conservation methods,
can further contribute to the overall
health and productivity of
agricultural systems.

2
 METHODOLOGY
Soil Analysis:

After we prepare the plot thoroughly, soil

samples were taken for lab test. The physical
and chemical properties of the soil were
studied. The NPK quantity in the soil was
analyzed before applying the synthetic NPK
fertilizer in different concentration.

Variety selected:

Mung bean [Vigna radiata (L.) R.

Wilczek] was selected as planting
samples for the current experimental
study. Vigna radiate is short duration
The research was conducted in the field of Chandaka Forest. It is situated leguminous plant with a maturity period
on the eastern fringes of Bhubaneswar, the capital city of Odisha. The of 60-70 days. It is a rich source of
geographical coordinates of Chandaka Forest are approximately 20.2966° protein and is commercially cultivated in
N latitude and 85.8610° E longitude. different parts of the world.

3
Soil samples collected from different location near the Deras Dam which comes under the buffering zone of Chandaka
Wildlife Sanctuary.
4
Field Parameter
After the selection of field, the layout was made and the experimentation took place from 02
December 2023 to 28 February 2024. The area designed as a Randomized Complete Block Design
(RCBD) with five treatments and three replications, with treatments allotted by lottery inside the
block.

S.N. Treatments Combination of NPK

1 T1 0:0:0

2 T2 10:10:10

3 T3 14:28:14

4 T4 19:19:19

5 T5 20:20:20

Microbial study:
For microbial study soil samples were cultured in different medium (i.e Nutrient Agar) medias after
10 days interval of fertilizer treatment.

5
6
RESULTS
Soil analysis:
The soil was light brown in color with low moisture content (9.4 ± 1.8) and the water holding
capacity was moderate. The soil samples had a fine sandy texture with 90 % sand content, 7 %
silt content, and very low clay (3.0%).
Microscopic view of soil under 10X lens (a) and 4X lens (b).

Soil Nutrient Quality Quantity

Organic Carbon (%) Low <0.5
Nitrogen (kg/acre) Low <280
Phosphorus (kg/acre) High >21
Potassium (kg/acre) Moderate 60-120

7
Germination of Vigna radiata:
After application of fertilizer in different ratio (T1, T2, T3, T4 and T5) we observed that the plants
had a faster germination and growth in T4 (NPK-19:19:19) in comparison to other. The longest
shoot (average 10.1 cm) was marked in T5 (NPK-20:20:20) but the plants showed delay in
germination as well as leaf proliferation. The longest root (average 3.6 cm) was marked in T3
(NPK-14:28:14) but the plants had slow germination and moderate shoot length (average 7.2 cm).
T1 (NPK-0:0:0) where the crude soil sample was used had faster germination (average 3 days)
than the plants grown on T2 (NPK-10:10:10), T3 and T4.

Treatment Days of Germination %

Length of Shoot (cm) Leath of Root (cm)
sprouting

T1 3 80.8 4.5 1.1

T2 4 66.1 6.3 1.8
T3 5 73.3 7.2 3.6
T4 3 86.7 9.7 1.2
T5 6 63.3 10.1 2.2

8
Growth of Vigna radiata on virgin soil from
buffering zone of Chandaka Forest treat with
different concentration of synthetic fertilizer
NPK (T1, T2, T3, T4 and T5).

The biomass of plants

treated with different
concentration of NPK

9
 Soil Microflora:

The various types of microbes observed in the soil sample of Chandaka Forest (a- spread plate
culture at different dilution, b- spread plate culture from soil sample without dilution, c- spread
plate culture from soil treated with T5 concentration of fertilizer, d- pure culture of two
prominent microbes isolated from soil treated with T5.)

10
CONCLUSION
From the above experimental study, we concluded that the soil samples that are collected
from the buffering zone of Chandaka Forest was highly rich in organic carbon and had 90 %
sand in it. Most of the flora in the area are tree species with few climbers which prevent the
sunlight to fall directly on the ground. The construction activities such as road development has
disrupted the ecosystem, providing an opportunity for invasive species to grow rapidly.

In the current study we also observed the effect of synthetic fertilizer (NPK) on the
germination of Vigna radiata. After application of fertilizer in different ratio (T1, T2, T3, T4 and
T5) we observed that the plants had a faster germination and growth in T4 (NPK-19:19:19) in
comparison to other. The longest shoot (average 10.1 cm) was marked in T5 (NPK-20:20:20)
and the longest root (average 3.6 cm) was marked in T3 (NPK-14:28:14). The biomass was
highest recorded (0.038 gm) in plants treated with T4 NPK.

11
THANK
YOU
12