Mosquito (Culicidae) Population Reduction Through Yeast

(SaccharomycesCerevisiae) with Sugar (Glucose)

in an Electronic Trap

RESEARCHERS:

Amanda Elizabeth D. Ostonal

Cassey Kiel C. Moncada
Janine Althea C. Fortunado
J-m H. Eviza
Jazmine I. Palma
Katrice A. Roldan
INTRODUCTION
• Mosquitoes in the school can disrupt the learning process as they
are a nuisance that discomforts students and teachers. Insects that
are dangerous in school settings are mosquitoes.
• The study focuses on investigating the use of yeast and brown
sugar as an effective mosquito attractant.
• It aims to protect the students, teachers, and other personnel of
MSEMSAT from mosquito bites/disturbances.
• The Department of Education (DepEd) has underscored the
imperative of intensifying efforts against dengue, particularly
within school settings where students are most susceptible.
INTRODUCTION
• Schools may unknowingly contribute to the spread of
mosquito-borne illnesses among students.
• Utilizing scientific developments is essential in fighting the
issues posed by vector-borne diseases
• The study highlights the potential of Saccharomyces
Cerivisiae (Yeast) and Glucose (brown sugar) as a natural
attractant that can lure mosquitoes inside the trap.
STATEMENT OF THE PROBLEM
1.) What doubts or worries could science teachers have about this
method's dependability and practical use compared to regular mosquito
control strategies?
2.) How do science teachers perceive the effectiveness of utilizing
Saccharomyces cerevisiae with glucose in an electronic trap for reducing
the population of Culicidae?
3.) Does the goal of decreasing the Culicidae population stand to gain
from incorporating novel biotechnological techniques?
4.) Does this innovation influence science instructors' professional
growth and intellectual processes in secondary school settings, especially
concerning rising technology and changing scientific knowledge?
Research Paradigm
Input - Process - Output
Gather data on science teachers' perceptions, concerns, and reservations about using
Saccharomyces cerevisiae with glucose in an electronic trap. This could involve surveys,
interviews, or focus groups.

Develop a procedure for crafting the Saccharomyces cerevisiae with glucose in an

electronic trap. This could involve specific steps for preparing the yeast and glucose
mixture and setting up the electronic trap.

Conduct data analysis using thematic coding techniques. Thematic coding involves
identifying recurring themes or patterns in the data and categorizing them accordingly.

Interpret the data to understand the perceptions, concerns, and reservations of science
teachers regarding the use of Saccharomyces cerevisiae with glucose in an electronic trap.

Saccharomyces cerevisiae with glucose in an electronic trap.

RELATED LITERATURES
This particular section will shed light to the literatures or studies that are related to
this research.

•Buser, C. C., et al. (2021). Scent of a killer: How could killer yeast boost its dispersal?
Ecology and Evolution 11 (11), 5809–5814. https://doi.org/10.1002/ece3.7534

•Stewart, T. A., et al. (2023). "Demonstration of RNAI Yeast Insecticide Activity in

Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on Aedes and
Culex Mosquitoes.". Insects 14 (12), 950. https://doi.org/10.3390/insects141209
RELATED LITERATURES
•Murindahabi, M. M., et al. (2022). A handmade trap for malaria mosquito surveillance
by citizens in Rwanda. PLOS ONE, 17(5). https://doi.org/10.1371/journal.pone.
0266714

•According to Maia et al. (2018), over 50% preferred to use attractive-toxic sugar baits
(ATSBs) indoors, despite instructions to place them outdoors.

•Dormont, L., Mulatier, M., Carraso, D., & Cohuet, A. (2021, March 16). Mosquito
attractants. SpringerLink. https://doi.org/10.1007/s10886-021- 01261-2
RESEARCH METHODS
Research Design: Grounded Theory Methodology

This study applies grounded theory to investigate the

effectiveness of yeast and sugar in an electro-mosquito trap. It
offers potential for grounded theorists in education to advance in
areas like curriculum studies and educational leadership. The
research aims to determine if yeast and sugar attract mosquitoes,
considering the presence of carbon dioxide within the trap.
RESEARCH METHODS
Research Population or Sample

This study involves (5) selected science teachers respondents

from Manuel S. Enverga Memorial School of Arts and Trades
to understand mosquito prevention beliefs, attitudes, and
behaviors, potentially using stratification to ensure
representation across departments.
RESEARCH METHODS
Research Instrument

The research instrument for this study involves conducting

interviews to gauge the reduction of the mosquito population in
MSEMSAT. The interview questions were carefully crafted in
alignment with the study's objectives, comprising seven (7)
questions.
 CHAPTER IV
PRESENTATION, ANALYSIS, AND
INTERPRETATION OF DATA
TABLE 1
Respondents' views on the impact of mosquito populations on the learning environment.

Themes
No. of Respondents Sample Extracts Descriptive Codes

Mosquito populations can carry diseases and cause discomfort, which Health threat on
R1 Learning of the students
can affect the health, focus, and learning of students. students

Destruction Health threat on

R2 It destroys the learning process, and it may cause illness.
students

In our school, there is nothing. Since the rooms are always clean and Health threat on
R3 Nothing
there is the proper path of water. students

The mosquito population is a health threat not only to the students but
Health threat on
R4 to everyone, making the learning environment not conducive to Health Threat
students
learning.

The emerging mosquito population has a considerable impact,

Health threat on
R5 especially on the learning environment, because it gives learners Environment
students
hindrances, especially in focusing on their studies.
TABLE 2
Perception of Science Teachers on the Effectiveness of Utilizing Saccharomyces Cerevisiae with Glucose in an Electronic Trap
No. of Respondents Sample Extracts Descriptive Codes Themes

Effective pests control

practices The use of yeast
R1 Slight Slight and sugar as a method to
minimize mosquito
populations

The use of yeast and

My only idea is that sugar and yeast release carbon dioxide that may cause the death of sugar as a method to
R2 can kill mosquitoes
mosquitoes. minimize mosquito
populations

The use of yeast and

sugar as a method to
R3 It is new to me, to be honest. new information
minimize mosquito
populations

The use of yeast and

sugar as a method to
R4 I am still waiting to hear about that. no idea
minimize mosquito
populations

The use of yeast and

I have yet to see a whole set-up, but regarding idea and familiarity, I am familiar with yeast sugar as a method to
R5 familiar
and sugar as attractive bait for minimizing mosquitoes. minimize mosquito
populations
TABLE 3
Incorporating Novel Biotechnological Techniques Decreasing the Culicidae Population
No. of Respondents Sample Extracts Descriptive Codes Themes

I think it would be adequate to implement yeast and sugar mosquito reduction methods in The effectiveness of
R1 our school, especially since I know that certain types of yeast can kill mosquitoes and, if positive impact the product can have a
implemented in our school, will have a positive impact. positive impact.

The effectiveness of
the product can
R2 If that project is successful, it will help to reduce the volume of mosquitoes in school. reduce mosquito
have a positive
impact.

The effectiveness of
R3 It can be implemented, but I suggest it during the rainy seasons. during rainy season the product can have a
positive impact.

The effectiveness of
Suppose this is tested and finds it beneficial and effective. In that case, this can be adopted
R4 economic the product can have a
since yeast and sugar costs are very economical and readily availabe. positive impact.

It might be implemented, but some help is needed. First is the area where the trap is
The effectiveness of
located; the size of the trap and the area of the location might affect each other. Another
R5 problem with location the product can have a
thing is that the nearby area without the trap set-up might be the new environment for positive impact.
mosquitoes, mainly because they will move away from the trap.
TABLE 4
Influence on Science Instructors' Professional Growth

No. of Respondents Sample Extracts Descriptive Codes Themes

Carbon dioxide emerges

Since some research discovered the effectivity of yeast and sugar mixture
R1 Proven by research as a key factor in
means it can be effective
attracting mosquitoes

Carbon dioxide emerges

R2 by releasing CO2 Carbon dioxide as a key factor in
attracting mosquitoes

Carbon dioxide emerges

I need to familiarize myself with the effectiveness of yeast and sugar in
R3 Effectiveness as a key factor in
attracting mosquitoes, but I hope it will be effective.
attracting mosquitoes

Carbon dioxide emerges

Yeast and sugar are effective mosquito baits because they contain carbon
R4 Carbon dioxide as a key factor in
dioxide; carbon dioxide is attractive to mosquitoes.
attracting mosquitoes

N/A N/A N/A

 CHAPTER V

SUMMARY, CONCLUSION AND

RECOMMENDATION
FINDINGS
1.) Science teachers may have doubts or worries about the dependability and practical use of utilizing Saccharomyces cerevisiae with
glucose in an electronic trap for mosquito control compared to regular strategies. They may question the long-term effectiveness, potential
environmental impacts, scalability, and cost-effectiveness of this novel biotechnological approach.
2.) Science teachers generally perceive the effectiveness of using yeast and brown sugar as promising for reducing the population of
Culicidae. They acknowledge the innovative nature of this technique and its potential to complement existing mosquito control methods.
3.) The goal of decreasing the Culicidae population stands to gain from incorporating novel biotechnological techniques such as utilizing
the prototype. This approach offers a targeted and environmentally friendly solution that could contribute to more sustainable mosquito
control practices.
4.) The innovation of using yeast and brown sugar as an mosquito attractant can influence science instructors’ professional growth and
intellectual processes in secondary school settings. It exposes them to cutting-edge biotechnological applications, encourages critical
thinking about emerging technologies, and fosters a deeper understanding of how scientific knowledge evolves over time.
 CONCLUSION
1) The target of this innovation is to create a novel machine that uses sugar and yeast to both
attract and eradicate mosquitoes on the premises of the Manuel S. Enverga Memorial School
of Arts and Trades. The idea offers a sustainable and environmentally friendly approach to
mosquito control in educational settings by combining natural attractants with efficient
trapping methods.
2) The research objectives were thoroughly stated in each chapter, along with a review of
related literature, a breakdown of the methodology used, and a data analysis that provides
insightful recommendations to strengthen safety protocols and foster a positive learning
environment for all the teachers, students, and other faculty personnel at MSEMSAT.
3) In essence, this study is a vital inquiry into the critical need for proactive measures to
protect the safety and health of individuals within educational institutions.
4) By descending into the utilization of natural resources as well as innovative complex
advancement, it unveils a comprehensive approach to mosquito control, presenting
promising addresses that transcend far beyond the immediate surroundings of MSEMSAT.
 RECOMMENDATION
1) The respondents should clearly understand the product's appearance and functionality.

2) It is recommended that enough respondents gather more perceptions on how the product, the
mosquito trap with yeast and sugar inside, will be effective in luring and minimizing the mosquito
population.

3) Regular awareness campaigns should be conducted to educate students and staff about mosquito-
borne diseases and prevention methods.

4) Install mosquito traps with yeast and sugar attractants in strategic areas of the school premises to
decrease mosquito populations.
REFERENCES:
Buser, C. C., Jokela, J., & Martin, Y. O. (2021). Scent of a killer: How could killer yeast boost its dispersal? Ecology and
Evolution 11 (11), 5809–5814. https://doi.org/10.1002/ece3.7534

Cappelli, A., Favia, G., & Ricci, I. (2021., January 21). Wickerhamomyces anomalus in mosquitoes: A promising yeast-based
tool for the "Symbiotic control" of mosquito-borne diseases. Frontiers. https://doi.org/10.3389/fmicb.2020.6 21605

Dormont, L., Mulatier, M., Carraso, D., & Cohuet, A. (2021, March 16). Mosquito attractants. SpringerLink.
https://doi.org/10.1007/s10886-021- 01261-2

Does blue LED light have a higher possibility to attract mosquitoes? (n.d.). https://typeset.io/questions /is-blue-led-light-has-a
-higher-possibility-to-attract -2fxl6sn3ab

Murindahabi, M. M., Takken, W., Hakizimana, E., Van, A. J., Poortvliet, P. M., Mutesa, L., & Koenraadt, C. J. (2022). A
handmade trap for malaria mosquito surveillance by citizens in Rwanda. PLOS ONE, 17(5).
https://doi.org/10.1371/journal.pone. 0266714

Stewart, T. A., Mysore, K., Njoroge, M. T., Winter, N., Feng, S. R., Singh, S., James, D. L., Singkhaimuk, P., Sun, L.,
Mohammed, A., Oxley, D. J., Duckham, C., Ponlawat, A., Severson, W. D., & Scheel, D. M. (2023). "Demonstration of RNAI
Yeast Insecticide Activity in Semi-Field Larvicide and Attractive Targeted Sugar Bait Trials Conducted on Aedes and Culex
Mosquitoes.". Insects 14 (12), 950. https://doi.org/10.3390/insects141209
Research
Defended!