“ Prepare and present“

Futun Arif (2314513) 

Aryam Alghamdi (2314015)
Taif Abdulhakeem (2314503)

Under the supervision of”“

Dr, Safa Abbas Sherfi
Principles of Sterilization
(ACST 212 )

King Abdul-Aziz University (KAU)

Applied Collage (AC)
Health Information Technology Department
(HIT) - Sterilization Technician Program
 Introduction

The low temperature steam formaldehyde (LTSF)

process is not a typical gas sterilization process but a
modified steam sterilization process .
Details of the sterilizer specifications
are available in the standard EN14180.
?WHAT IS FORMALDEHYDE

It is a colourless gas, but is normally distributed as a solution

(generally referred to as formalin), and is known to most people
within hospitals as an important disinfectant, which has, been in use
since the late 1800s.

The principal use in hospitals is for

fixation of tissues.
Stages of Low-Temperature Steam and Formaldehyde

Stage- 1: Pre-treatment
* Conditioning Phase
Stage- 2: Formaldehyde admission
* Formaldehyde saturation
Stage- 3: Sterilization
Stage- 4: Post-treatment
* (A) Formaldehyde desorption
Stage- 5: Drying
 What is full sterilization

Sterilization may be defined as the statistically complete destruction of all

microorganisms including the most resistant bacteria and spores.
Sterilization may be defined as the statistically complete destruction of all
microorganisms including the most resistant bacteria and spores
 What is dry heat sterilization
Dry heat sterilization is one of the most practical and preferable forms of 
sterilization, using blown hot air to eliminate or deactivate all forms of life inside the
chamber of an industrial oven
Typically, exposure to 100°C for 20 minutes on 3 successive days is required. The * 
principle is that the first exposure kills all spores and vegetative bacteria. If they
germinate, they will be killed in the subsequent exposures
 ?What are the 7 steps of sterilization

There are seven primary methods for medical device sterilization. These methods are 
steam sterilization, radiation sterilization, dry heat sterilization, sterilization by filtration,
gas sterilization (such as ethylene oxide sterilization), vapor sterilization, and liquid
. sterilization
 Conclusion
? What is the purpose of sterilization 

It is the process in which all microorganisms , such as fungi, bacteria and viruses, including bacterial
.spores, are eliminated from the materials to be sterilized
While disinfection and sterilization are two different types of decontamination process, they aim .
to eliminate enough germs so that the risk of infection or illness is very low . In short, disinfection is the
process of destroying or reducing harmful microorganisms while sterilization is the process of killing all
.microorganisms
&End