STAGES OF ERYTHROPOEISIS

Proerythroblast or megaloblast
● first cell derived from the stem cell

● large nucleus & occupies the cell completely.

● two or more nucleoli & a reticular network.

● No hemoglobin.

● It multiplies several times and finally forms the basophilic

erythroblast or early normoblast.
 Basophil erythroblast or early normoblast
● little smaller.
● The nucleoli disappear and condensation of chromatin
network occurs.

Polychromatic erythroblast or Intermediate normoblast

● smaller than early normoblast and the nucleus is still
present.
● Chromatin network shows further condensation.
● The hemoglobin starts appearing.
● stains with both acidic and basic dye hence called as
polychromophilic erythroblast.
Orthochromatic erythroblast or Late normoblast

● Nucleus becomes very small with very much condensed

chromatin network and it is known as ink spot nucleus.
● quantity of hemoglobin increases.
● the nucleus disintegrates and disappears.

Reticulocytes
● It is also called as immature RBC.
● It is slightly larger than matured RBC.
● The cytoplasm contains the reticular network hence the cell
is called reticulocyte.
Matured erythrocyte

● The reticular network disappears

● has hemoglobin but no nucleus.

● Requires seven days for the development and maturation of

RBC from pro-erythroblast.

● Requires five days upto the stage reticulocyte.

● Two more days to become the matured RBC.