PERIODONTAL LIGAMENT

CELL SHEET

Agustina Putri
6478505032
PERIODONTAL TISSUE

Cho, Y. D., et al. (2021). Periodontal wound

healing and tissue regeneration: A narrative
review.
 PERIODONTAL LIGAMENT

Connecting cementum
Soft connective tissue Type 1 collagen to gingiva and alveolar
bone

Form, maintain, and

Mostly consist of repair the alveolar
fibroblast cells bone and cementum

Mortazavi, H., & Baharvand, M. (2016).

Review of common conditions associated with
periodontal ligament widening.
PERIODONTAL DISEASE

Sedghi, L. M., et al. (2021). Periodontal

disease: The good, the bad, and the
unknown.
 TREATMENTS
Scallind and Root
Non-Surgery Planing

Periodontal Periodontal Flap

Surgery
Disease Treatment

Cell-based
GTR and GBR
treatment

Raju, R., Oshima, et.al., (2020). Three-

dimensional periodontal tissue regeneration
using a bone-ligament complex cell sheet.
CELL-GUIDED PDL REGENERATION

Ward, E. (2022). A review of tissue

engineering for periodontal tissue
regeneration.
PERIODONTAL LIGAMENT STEM CELLs

Trypsin EDTA or
Type 1
Collagenase

Iriarte, C. G. T., et al. (2017). Isolation of

periodontal ligament stem cells from extracted
premolars. Simplified method.
 LIMITATION OF PDLSc
Immune response

Low survival and grown rate of the cells in

vitro before implantation
Cell
Inability of injected cells to attach to the site of Sheet
implantation, and

Lack of vascularization or difficulty in

revascularization in the site of interest
CELL SHEET

Chen, G., et al. (2015). Application of the cell

sheet technique in tissue engineering.
HOW TO GET CELL SHEET

Bousnaki, M., et al. (2022). A review of in vivo

and clinical studies applying scaffolds and cell
sheet technology for periodontal ligament
regeneration.
 APPLICATION OF CELL SHEET

3D
Coating
Structure

Pellet Graft

Bousnaki, M., et al. (2022). A review of in vivo and clinical studies applying scaffolds and cell sheet technology for periodontal ligament regeneration. Biomolecules, 12(3), 435.
APPLICATION OF CELL SHEET FOR
PERIODONTAL REGENERATION
 BASELINE 6 months post op 75 months post op

*Patient 1 : 33 years old

BASELINE 6 months post op 35 months post op

*Patient 2 : 64 years old

BASELINE 6 months post op 12 months post op

C
*Patient 3 : 55 years old
LIMITATION OF CELL SHEET