VERIFICATION OF BLUE MOLIBDENUM METHOD IN

DETERMINATION PHOSPHATE LEVELS IN SHRIMP WASTE WATER

AS A TEST LAMPUNG NATURAL ZEOLITE ADSORPTION

(Research Result)
By

Merry Yanti
1717011092

DEPARTMENT OF CHEMISTRY
FACULTY OF MATHEMATICS AND NATURAL SCIENCES
UNIVERSITY OF LAMPUNG
2022
 BACKGROUND OF RESEARCH

PO43- (aq) + 12 MoO42- (aq) + 27

H+ (g) → H7(P(Mo2O7)6) (aq)

H7(P(Mo2O7)6) (aq)+ 10H2O(l) +

H7(P(Mo2O7)6) (aq) + C6H8O6 (s)

 Research Benefits of
Purposes Research

01 Verify the method of measuring phosphate

levels in shrimp pond wastewater using 01 Support programs and
the Molybdenum-Blue method with activities in maintaining
parameters including linearity, precision, marine ecosystems.
accuracy, limit of detection (LoD), and
limit of quantitation (LoQ).

Using a verified method to analyze Controlling the water quality

02 phosphate levels in shrimp pond 02 of shrimp ponds to keep
wastewater by adsorption method using them awake.
natural zeolite before and after treatment.
 Research Methods
Time and Place

This research was conducted in July 2022 – September 2022, a series of research and characterization of
adsorbents using Scanning Electron Microscopy- Energy Dispersive X-Ray (SEM-EDX), and analysis of
phosphate content with UV-Vis Spectrophotometry was carried out at the Integrated Laboratory and the
Center for Technological Innovation ( UPT-LTSIT), Lampung University .
The materials used in this study were Natural zeolite 100 mesh size from Katibung area, Campang Tiga, South
LampungTools and Materials Aquades, KH2PO4, AlCl3.6H2O, HCl, H SO , ((NH )6Mo O .4H O),
Regency,Wastewater, 2 4 4 7 24 2
(K(SbO)C4H4O6.1/2H2O), Indikator Fenolftalin and C6H8O6 .
The tools used in this study glassware, erlenmeyer, analytical scales, oven, centrifuge, shaker, SEM-EDX
(Scanning Electron Microscope, Energy Dispersy X-ray), UV-Vis spectrophotometry Carry 100 Conc.

The materials used in this study were Natural zeolite 100 mesh size from Katibung area, Campang
Tiga, South Lampung Regency,Wastewater, Aquades, KH 2PO4, AlCl3.6H2O, HCl, H2SO4,
((NH4)6Mo7O24.4H2O), (K(SbO)C4H4O6.1/2H2O), Indikator Fenolftalin and C6H8O6 .
 Research Procedure

1 2 3 4 5

METHODE CHARACTERIZATIO
PREPARATION ZEOLIT TREATMENT ANALYSIS
VERIFICATION N
Results and Discussion
METHODE VERIFICATION

Sample
Repitition Absorbance Actual concentration (ppm)

1 0, 2151 1,842
2 0,2156 1,848
3 0,2144 1,833
4 0,2154 1,846
5 0,2166 1,861
6 0,2160 1,853
7 0,2161 1,854
8 0,2163 1,857
Picture. Kurva Linieritas Standar Phospate Average 1,849
SD 0,0089
% RSD 0,4813

Table. Precision results of shrimp pond wastewater samples

METHODE VERIFICATION

Repetition Absorbance Actual concentration (ppm) % Recovery

1 0,2151 1,842 108,4
2 0,2156 1,848 109,6
3 0,2144 1,833 106,6
4 0,2154 1,846 109,2
5 0,2166 1,861 112,2
6 0,2160 1,853 110,6
7 0,2161 1,854 110,8
8 0,2163 1,857 111,4
Average 109,85
Maximum 111,4
Minimum 106,6

Picture. The results of the accuracy of shrimp pond wastewater samples.

 Zeolite Alam Treatment

A B C

Figure (a) 100 mesh size natural zeolite, (b) natural zeolite
washing, (c) dried natural zeolite.

A B C

Figure (a) immersion of zeolite with HCl, (b) washing of zeolite

with distilled water, (c) activated zeolite.
 SEM-EDX CHARACTERIZATION

Figure SEM analysis (a)

Micrograph of natural zeolite
with a magnification of 10,000 x.
(b) Al modified zeolite
micrograph with a magnification
of 10,000 x.

(A) (B)

Image of EDX Test Results (a) natural

zeolite. (b) Zeolite is treated
 Elemen Wt% At% Elemen Wt% At%
O 52,04 65,64 O 51,31 64,75
Si 40,06 28,79 Si 41,58 29,89
Al 6,31 4,72 Al 6,84 5,12
Ca 0,74 0,37 Ca - -
K 0,70 0,36 K - -
Mg 0,74 0,12 Mg - -

Table. Analysis of natural zeolite elements Table. Analysis of zeolite elements after being treated.

SURFACE AREA
 1.4
Shrimp Pond Wastewater
Konsentrasi Fosfat (mg/L)

1.2
1 Adsorption Test
0.8
0.6
0.4
0.2
0 Figure and Table. Phosphate absorption graph in
0 0.5 1 1.5 2 2.5

Berat Zeolit (gram)

Natural Zeolite before and after being treated .

zeolit alam zeolit setelah diberi perlakuan

Phosphate Adsorption Concentration (mg/L) Percentage Absorption (%)

No Zeolite Natural Zeolite After Natural Zeolite After
Weight Zeolite treatment Zeolite treatment
1 0 1,3 1,3 0 0
2 0,5 1,24 1,17 4,83 11,11
3 1 1,14 1,05 14,03 17,11
4 1,5 1,08 0,97 20,37 32,65
5 2 1,01 0,90 28,71 44,44
6 2,5 0,94 0,84 38,29 52,94
 Conclusions

1. Method of verification of the analysis of phosphate ion content in shrimp pond wastewater
using the Molybdenum-blue method. has met the acceptance requirements.
2. This method can be used to determine the phosphate content in shrimp pond wastewater with
a detection limit of 0.116 mg/L and a quantification limit of 0.389 mg/L.
3. Based on the research that has been done, it can be concluded that the treated zeolite
undergoes structural changes that cause its surface area to increase so that it can increase its
absorption capacity and can reduce phosphate levels in shrimp pond wastewater.

Suggestions

Based on the research that has been done, the method that has been carried out can be used to
analyze phosphate in other wastes, but further research needs to be done.
Thanks you