Comparative studies on body composition and mineral

status of selected herbivorus and carnivorus fishes in

riverine system of Punjab
Mahnoor Zaidi
224239
M. Phil Zoology (M)

Supervisory Committee
Supervisor Prof. Dr. Salma Sultana
Member -I : Dr. Syed Makhdoom Hussain
Member -II : Prof. Dr. Tayyaba Sultana

Department of Zoology
Government College University Faisalabad
 Need for the Project
Essential components
Most abundant amino acids
Maintenance of are glutamic acid, aspartic
good health acid, and lysine and enrich
omega 3 fatty acids

Proximate composition, a
Good source good indicator of fish
of minerals physiology (Meat and
nutrient)

Role in maintaining
body functions, bones The protein upto 30% , Moisture ranked first,
formation fat upto 25%, and the Protein ranked second
moisture upto 80% of and Fat ranked third
body weight ( Ahmed et al, 2022)
(Duran et al., 2020 , Ozden and Erkan, 2018)
 Experimental Fishes

Labeo rohita
• Rahu
• Herbivore
• Column feeder
• Consumer Choice Catla catla
• Thaila
• Herbivore
• Surface Feeder Cirrihinus mrigala
• Fast growing • Mori
• Herbivore
• Bottom Feeder

(Shakir et al., 2019)

 Wallago attu
• Malli
• Carnivores
Chana marulius • stable market price
• Snakehead
• Carnivores
Sperata seenghala • Very tasty
• Khagga / Singhara in Pakistan • Nutritional value
• Carnivores
• Good source of lean meat
• and trace elements, especially
zinc and iron. ( Zia et al. 2017 )
 There are significant differences with respect to

Hypothesis
body composition and mineral status of herbivorous
and carnivorous fish species in riverine system and
local hatcheries of Punjab.
 To Evaluate the
Proximate
Composition of
Riverine Fishes
Aims and
Objectives To Determine
mineral status of
Herbivorous and
carnivorous Fishes

To analyze fatty
acid and amino acid
profile of different
Fishes
 Methodology

Proximate
Sampling of fish analysis and
Study Area
species estimation

Statistical
Mineral Fatty acid Profile
analysis
estimation
 Study Area

River Indus,
River Ravi, Head
Chasma head,
Balloki, lahore
Mianwali.
5 Rivers of Punjab,
Pakistan

River Chenab,
River Sutlej,
Head Trimmu,
Head Panjnand,
Jhang
Muzafargarh

River Jehlum,
Head Trimmu,
Jhang
Sampling of fish species
Fish samples will be collected

L. rohita

3 samples of each
Herbivorous fish
species from each C. Catla
river

C. mrigala

W. attu
3 sample of
each
S. seenghala Carnivorus fish
species from
each river
C. marulius
 Proximate Analysis
Protein content
by Kjeldhal
Apparatus
Moisture content
by Oven-Drying
method

Ash content
by Electric
Fat content by furnance
Soxhlet
Apparatus

(AOAC,2005 , Barua et al. 2021)

 Calculations

(Weight Loss during Oven Drying / Original Wet Body

Moisture (%) = Weight of Sample Taken) x 100

(Incinerated Sample Weight / Original Wet Body

Ash % = Weight of Sample Taken) x 100

(Weight of residue left in Bottle / Original Wet

Fat % = Body Weight of Sample Taken) x 100

(AOAC,2005 , Barua et al. 2021)

 Amino acid and Fatty acids analysis

The amino acids will be

determined by using the
Aracus Amino Acid Analyzer
.
Fatty acids will be analyzed by
gas chromatography (GC)
(AOAC, 2005)
Mineral estimation

Mineral estimation will be

done by Atomic absorption
spectroscopy
(AOAC, 2005)
 Statistical Analysis

• One-way analysis of variance

(ANOVA)
• and Co-Stat computer software
will be used.

(Snedecor et al., 1991; Steel et al.,

1996).
 Naz et al.
(2022)
Hussain
N.Khan
et al. ( 2018 )
et al.
( 2018 )

REVIEW OF Khalid
LITERATURE
Khalid et al. et al. (2022)
(2018)

M.Y. Laghari Habib et al.

et al. ( 2019 ) (2021)
Ahmed et
al. ( 2020 )

More than 30 articles are cited in Synopsis for the current research
 References
• Ahmed, I., & Sheikh, Z. A. (2017). Study on the seasonal variation in the chemical composition, hematological profile, gonado-somatic index
and hepato-somatic index of snow trout, Schizothorax niger from the freshwater Dal Lake, Kashmir. American Journal of Food Technology,
12(1), 1-13.
• Ahmed, I., Jan, K., Fatma, S., & Dawood, M. A. (2022). Muscle proximate composition of various food fish species and their nutritional
significance: A review. Journal of Animal Physiology and Animal Nutrition, 106(3), 690-719.
• Barua, P., Pervez, M. A., Sarkar, D., & Sarker, S. (2021). Proximate biochemical composition of some commercial marine fishes from Bay of
Bengal, Bangladesh. Mesopotamian Journal of Marine Sciences, 27(1), 59-66.
• Begum, M., Akter, T., & Minar, M. H. (2021). Analysis of the proximate composition of domesticated stock of pangas (Pangasianodon
hypophthalmus) in laboratory condition. Journal of Environmental Science and Natural Resources, 5(1), 69-74.
• Hadyait, M. I., Ali, A., Bhatti, E. M., Qayyum, A., & Ullah, M. Z. (2018). Study of Proximate Composition of Some Wild and Farmed Labeo
rohita and Cirrhinus mrigala Fishes. PSM Biol. Res, 3(1), 34-38.
• Jan, K., Ahmed, I., & Dar, N. A. (2021). Haematological and serum biochemical reference values of snow trout, Schizothorax labiatus habiting
in river Sindh of Indian Himalayan region. Journal of Fish Biology, 98(5), 1289 ‐1302.
• Li, X., Bi, X., Wang, S., Zhang, Z., Li, F., & Zhao, A. Z. (2019). Therapeutic potential of ω-3 polyunsaturated fatty acids in human
autoimmune diseases. Frontiers in Immunology, 2241.
• Parveen, I., & Gaikwad, J. M. (2018). Diversity of fish parasites from fresh water fishes of Masooli Reservoir of Parbhani district.
International Journal of Scientific and Research Publications, 8, 371-376.
• Rani, P. S. C. H. P. D., Kumar, V. P., Rao, R. K., & Shameem, U. (2016). Seasonal variation of proximate composition of tuna fishes from
Visakhapatnam fishing harbor, east coast of India. International Journal of Fisheries and Aquatic Studies, 4(6), 308-313.
• Zhang, L., Yang, Z., Yang, M., Yang, F., Wang, G., Liu, D., ... & Wang, Z. (2022). Copper-induced oxidative stress, transcriptome changes,
intestinal microbiota, and histopathology of common carp (Cyprinus carpio). Ecotoxicology and Environmental Safety, 246, 114136.
• Zia, M.A., Qurat-ul-Ain, Abudullah S., Abbas, K. and Ahmed, I. (2017). Sublethal impacts of heavy metals on antioxidant enzymes and
biochemical parameters in rohu (Labeo rohita). Iranian Journal of Fisheries Sciences, 16(2), 668-683.
Thank you for
listening

Any
Suggestion??
?