Maaza Ahmad

2021-ag-227
M.sc. (hons.) Food Technology
National Institute of Food Science
University of and Technology
Agriculture
Faisalabad
APPLICATION OF POLYLACTIC
ACID AS ETHYLENE
SCAVENGER IN ACTIVE
PACKAGING OF GUAVA
 01 Need for the project

02 Hypothesis

03 Review of literature
ROAD MAP
04 Objectives

05 Materials and Methods

06 References
 • Guava fruit softens faster due to its
climacteric nature
• Soft rot of guava caused by
Rhizopusstolonipher during storage

NEED OF THE • Guava fruit is highly susceptible to

PROJECT chilling injury and mechanical damage
• Post harvest losses are 20-40%

(Rawan et al., 2017)

 NEED OF THE PROJECT

• Active packaging a new concept in response to current

consumption and market trends
• Novel concept in active packaging as compare to classical
methods

(García-García et al., 2013)

 • PLA coated with thin LDPE film may adsorb ethylene
allow to reduce water vapor and prevent microbial
recontamination in package
HYPOTHESIS
 REVIEW OF LITERATURE
Study
Hydrophobic properties of
cardboard coated with poly
lactic and ethylene scavengers
Poly lactic acid film
incorporated with marigold
flower extract(Tagetes erecta)
intended for fatty-food
application
Results Citation
PLA 2% alone or with C 1.5% or (García-García et al.,
with 1% of SP were the most 2013)
effective in increasing the water
barrier and water adsorbent
properties of cardboard and
improved humidity behavior
PLA2M did retarded the (Samsudin et al.,
oxidation of soybean oil for 15 to 2014)
25 days Shelf life extended but
PLA2M did not show any effect
in prolonging the freshness of
soybean oil
 REVIEW OF LITERATURE
Study
Active packaging of
cardboard to extend the
shelf life of tomatoes
Properties of poly(lactide)
coated paperboard for the
use of 1-way paper cup
Results Citation
Application of PLA inside (García-García et al., 2013)
cardboard lowers the
ethylene and extended shelf
life of tomatoes preventing
microbial contamination
TS, WVP, WA, CA and wet (Rhim and Kim, 2009)
TS were improved by PLA
coating paperboards coated
with up to 3 w/v% PLA
coating solution were very
effective in water barrier
properties of paperboard
 REVIEW OF LITERATURE
Study
Potential use of poly(lactic
acid) nano fiber mats as
nano-sachet in post harvest
of climacteric fruits and
vegetables
Postharvest life of
guava(psidium guajavaL.)
varieties as affected by
storage intervals at room
temperature
Results Citation
PLA nanofiber mats showed (Miranda et al., 2021)
an excellent barrier
properties ripening process
delayed effective ability in
ethylene scavenging and
moisture absorption for
preventing postharvest
quality of tomatoes
Kkl (Rawan et al., 2017)
 • Utilization of PLA as active scavenger to control
ethylene concentration of guava fruit

• Replacement of perforated films with thin LDPE films

OBJECTIVES to prevent microbial contamination

• To extend the shelf life and delay respiration process

 MATERIAL AND METHOD

Guava

Micro corrugated cardboard tray

Material
LDPE film
required

PLA

Chloroform
(García-García et al., 2013)
 TREATMENT PLAN

Treatments PLA solution% LDPE

T0 0% Without film

T1 2% Thick

T2 2% Thin

T3 3% Thick

T4 3% Thin

Storage study will be of 25 days at room temperature of 20 ± 2°C

 PROCEDURE
Procurement of Formulation of Application of
fresh guava PLA solution solution

Thickness
Filling trays Wrapping trays
measurement of (García-
with sample with LDPE film García et al.,
PLA lining
2013)
 PRODUCT ANALYSIS

Physicochemical
Analysis

Statistical
Analysis

Microbial analysis
 PHYSICOCHEMICAL ANALYSIS

 Weight loss
 MC
 Color determination
 Firmness
 pH
 TSS
 Ethylene determination
 MICROBIAL STATISTICAL
ANALYSIS ANALYSIS

 Decay loss  Data will be collected and furthure

analyzed
 REFERENCES

 García-García, I., A. Taboada-Rodríguez, A. López-Gomez and F.

Marín-Iniesta. 2013. Active Packaging of Cardboard to Extend the
Shelf Life of Tomatoes. Food Bioprocess Technol. 6:754–761.

 Miranda, K.W.E., J.D. Bresolin, C.V.L. Natarelli, S.D. Benevides,

M. do S.R. Bastos, L.H.C. Mattoso and J.E. de Oliveira. 2021.
Potential use of poly(lactic acid) nanofibers mats as Nano-sachets
in postharvest of climacteric fruits and vegetables. J. Appl. Polym.
Sci. 138:1–10.
 REFERENCES

 Rawan, S., F. Bibi, N. Khan, A.M. Khattak, Z. Shah, A. Iqbal, M.

Alamzeb, S.U. Haq, A. Kamal, F.A. Shah, A. Naeem and W. Ali.
2017. Postharvest Life of Guava (PsidiumguajavaL.) Varieties
as Affected by Storage Intervals at Room Temperature.
Pakistan J. Agric. Res. 30:155–161.

 Rhim, J.W. and J.H. Kim. 2009. Properties of poly(lactide)-

coated paperboard for the use of 1-way paper cup. J. Food Sci.
74:105–111.
 REFERENCES

 Samsudin, H., H. Soto-Valdez and R. Auras. 2014.

Poly(lactic acid) film incorporated with marigold
flower extract (Tagetes erecta) intended for fatty-
food application. Food Control. 46:55–66.
 Taboada-Rodríguez, A., I. García-García, R. Cava-
Roda, A. López-Gómez and F. Marín-Iniesta. 2013.
Hydrophobic properties of cardboard coated with
polylactic acid and ethylene scavengers. J.
Coatings Technol. Res. 10:749–755.