NMR(Nuclear Magnetic Resonance

Discovery :

 In 1946, NMR was co-discovered by Edward Purcell at Harvard and Felix Bloch at
Stanford University.
 The discovery first came about when it was noticed that magnetic nuclei, such as 1H
and 31P were able to absorb radio frequency energy when placed in a magnetic field of a
strength that was specific to the nucleus. Upon absorption, the nuclei begin to resonate
and different atoms within a molecule resonated at different frequencies.
 This observation allowed a detailed analysis of the structure of a molecule. Since then,
NMR has been applied to solids, liquids and gasses, kinetic and structural studies.
Introduction to NMR :

 Nuclear magnetic resonance spectroscopy, most commonly known as NMR

spectroscopy or magnetic resonance spectroscopy (MRS), is
a spectroscopic technique to observe local magnetic fields around atomic nuclei.
 It is a spectroscopy technique that is based on the absorption of electromagnetic radiation
in the radiofrequency region 4 to 900 MHz by nuclei of the atoms.
 Over the past fifty years, NMR has become the preeminent technique for determining the
structure of organic compounds.
 Of all the spectroscopic methods, it is the only one for which a complete analysis and
interpretation of the entire spectrum is normally expected.
 Basic Principle :

 Many nuclei have spin, and all nuclei are electrically

charged, according to the NMR principle. An energy
transfer from the base energy to a higher energy level is
achievable when an external magnetic field is supplied.
 All nuclei are electrically charged and many have spin.
 Transfer of energy is possible from base energy to higher
energy levels when an external magnetic field is applied.
 The transfer of energy occurs at a wavelength that coincides with the radio
frequency.
 Also, energy is emitted at the same frequency when the spin comes back to its
base level.
 Therefore, by measuring the signal which matches this transfer the processing
of the NMR spectrum for the concerned nucleus is yield.
 Nuclear spin is related to the composition of an element’s nucleus. Nuclei
containing an even number of both protons and neutrons have 0 nuclear spin
and cannot undergo NMR (e.g., 4He,12C,16O). Nuclei with an odd number of
protons and/or neutrons display nuclear spin and can experience NMR
(e.g., 1H, 2H, 14N, 17O).
 These nuclei behave like tiny spinning magnets and can interact with an external magnetic field.
Spinning nuclei also create their own magnetic field that can interact with other nuclei having spin.
 During NMR, an applied magnetic field excites nuclei having different magnetic moments across
various energy levels.
 After absorbing a characteristic radiofrequency, the excited-state nuclei return to lower energy states
by transferring energy to the surrounding environment. When energy is transferred to other atoms or
the solvent, the relaxation process is called ‘spin-lattice relaxation’. If energy is transferred to
neighboring nuclei at the same energy level, the process is termed ‘spin-spin relaxation’.
 These two relaxation processes are characterized by time constants: spin-lattice relaxation time (T 1)
and spin-spin relaxation time (T 2), which are responsible for the resulting NMR spectrum.
Instrumentation of NMR :

 This instrument consists of nine major parts. They are discussed below:
 Sample holder – It is a glass tube which is 8.5 cm long and 0.3 cm in diameter.
 Magnetic coils – Magnetic coil generates magnetic field whenever current flows
through it
 Permanent magnet – It helps in providing a homogenous magnetic field at 60 – 100
MHZ
 Sweep generator – Modifies the strength of the magnetic field which is already
applied.
 Radiofrequency transmitter – It produces a powerful but short pulse of the
radio waves.
 Radiofrequency – It helps in detecting receiver radio frequencies.
 RF detector – It helps in determining unabsorbed radio frequencies.
 Recorder – It records the NMR signals which are received by the RF detector.
 Readout system – A computer that records the data.