Name- krishnakanta sau

student code – BWU/BBT/21/061

Subject – plant biotechnology
presentation topic – shoot tip culture
 What is shoot tip culture
• Shoot tip culture may be described as the culture of terminal (0.1-1.0 mm) portion of a
shoot comprising the meristem (0.05-0.1 mm) together with primordial and developing
leaves and adjacent stem tissue.
Why shoot tip Culture

➤Virus free plants

➤No cell differentiation
➤ virus cant move from cell to cell
➤Intense metabolic activity
➤No vascular system
 PROTOCOL

Sterilization: Clean and sterilize shoot tips.

Prepare Medium: Create a sterile nutrient medium.
Initiate Culture: Transfer sterilized shoot tips onto the medium.
Incubate: Grow cultures in controlled conditions.
Subculture: Transfer growing shoots to fresh medium periodically.
Plantlet Development: Transfer developed shoots to soil.
1. Hardening Off: Gradually expose plantlets to outdoor conditions.
 STAGES OF SHOOT TIP CULTURE
Stage 1 is the culture establishment stage
• when explant may develop into single shoot or multiple shoots.
• At this stage explant are supplements with cytokinin like BA, kinetin and
2iP.
In stage 2
• the objective is to multiply the propagule and for this axillary shoot
proliferation is followed as it maintains higher genetic stability.
The stage 3
• purpose is regeneration of adventitious roots from the shoots obtain in
stage 2
• Numerous studies have indicated that NAA is followed by IBA, IAA,
2,4-D and other auxins are used for induction of root generation
Factors affecting Shoot tip culture

Genotype: Different plant species and varieties respond differently to shoot tip culture techniques.
Nutrient Medium: The composition of the growth medium, including types and concentrations of nutrients,
hormones, and supplements, affects growth and development.
Hormones: Growth regulators such as auxins and cytokinins are crucial for initiating and promoting shoot
growth in culture.
Temperature: Optimal temperature ranges promote tissue growth and development while extreme
temperatures can inhibit growth or cause tissue damage.
Light: Proper light intensity and quality are essential for photosynthesis and regulating plant growth and
development in culture.
pH: The pH level of the growth medium affects nutrient availability and metabolic processes in the cultured
tissues.
 APPLICATION

Propagation: Rapid multiplication of disease-free plants from a small

amount of starting material.
Germplasm Conservation: Preservation of elite plant varieties and
endangered species.
Genetic Transformation: Introduction of desired traits or genes into plants
for crop improvement.
• Disease Elimination: Production of pathogen-free plants through shoot tip
culture techniques.
 Acknowledge

I would like to express my special thanks of gratitude to my teacher Dr.

Prasant Shukla as well as our principal Dr. Sayantan Ghosh Who gave me
the golden opportunity to this presentation topic “Shoot tip culture ”which
also helped me in doing a lot of Research and i came to know about so many
new things I am really thankful to them.

THANK YOU