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Seed Production 1 1
Seed Production 1 1
CPT
06102
TUTOR; MADAM FAITH WILILO
PRODUCTION OF SEED CROPS
• Credit hours= 14
Assessment:
• At least 3 tests
• ……. Quizzes/assignment
• Semester examination
PRODUCTION OF SEED CROPS..
• Content;
a)Definition-seed
b)Attributes of quality seed
c) Plant breeding technologies in seed
production
d)Techniques to develop and maintain seed
varieties
e)Seed production systems in Tanzania
PRODUCTION OF SEED CROPS..
f) Seed production in seed crops
Site selection and land preparation, sowing seed
and transplant seedlings, gap filing and thinning,
weeding, erthing up, fertilizer application, pest
control, detussling, emasculation, harvesting,
packing and storing seed.
INTRODUCTION
Definition of seed
• In agriculture, seed is broadly defined as a
living thing used to produce a plant. Or
• is the part of the plant which is used or
intended to be used for propagation.
• They are the living organisms, which carry the
genetic properties of a crop necessary for the
propagation of the plant species.
Introduction…
• Seed is a fertilized mature ovule consists of a
miniature plant (embryo) and food reserves.
• Generally, the term seed refer to botanical
seed as well as vegetative materials. In other
words seed is part of a plant from which a
new plant of the same kind can grow.
Introduction…
• Types of seed
-true seed and
-vegetative propagation materials.
• True seed: cereals, grasses, legumes and most
vegetables seed , Some fruit trees such as mangoes
also produce true seeds
• 2.2 RESPIRATION
• Respiration is a process of oxidizing food to release energy
inside cells.
• respiration in plants involves using the sugars produced
during photosynthesis plus oxygen to produce energy for
plant growth, takes place in the mitochondria of the cell
• C6H12O6 + 6O2 → 6CO2 + 6H2O + 32 ATP (energy)
• Respiration increases dramatically as the seed sprouts.
Seed physiology…
• 2.2.2 Importance of respiration
• Cellular respiration converts the energy in
glucose to energy in the form of ATP which is
used to power physiological processes like seed
germination and growth.
• Aerobic respiration returns CO2 to the
atmosphere to be used again in photosynthesis
Seed physiology…
• Seed dormancy is a situation whereby viable
seeds fail to germinate in the presence of
favourable environmental conditions.
• It is a biological mechanism that provides
protection against germination when
environmental conditions may not be
favourable for seedling growth.
Seed physiology…
• A dormant seed is a live seed that is unable to
germinate in a specific period of time under a
combination of environmental factors that are
normally suitable for germination.
Some causes of seed dormancy are:
• a) Hard seed coat
• b) Embryo dormancy
• c) Immature embryo- the embryo is not fully
developed when the seed is dispersed
• d) Chemical inhibitor
Seed physiology…
• 2.3.1 Types of dormancy
• i. Quiescent(resting phase) seed dormancy -
even a non-dormant or quiescent seed has a
unique ability to revert to a dormant state
under stressful conditions. Dormancy is
desired in the wild, where plants depend
entirely on nature for survival.
Seed physiology…
ii. Structural dormancy is imposed via seed coat,
which prohibits the entry of water, air, outward
diffusion of possible endogenous germination
inhibitors, as well as through mechanical
restriction of embryo growth.
Species with hard, impervious seed coverings occur
in trees such as Acacia species. To help such
seeds to germinate, mechanical methods were
employed to loosen the seed covering by
cracking the seed coat.
Seed physiology…
iii. Physiological dormancy (embryo dormancy) occurs
when the embryo requires a special treatment to
induce it to start active growth.
• In some plant species, embryo dormancy can be
broken by a certain quality of light. For example,
exposure of lettuce seeds to red light (about 660 nm)
induces germination, but far red light (730nm) inhibits
it.
• It was indicated long years ago that if lettuce seeds
received red light after exposure to far red light,
germination occurred and the germination of seeds of
this species can occur only as long as the last treatment
before sowing was red light.
Seed physiology…
Methods of breaking seed dormancy;
i. Scarification: Scarification is when the seed coat
is rubbed with something such as sandpaper. This
makes the seed coat thinner so water can enter
more easily. This method is best for smaller seeds
that are hard to cut. To do this easily you can
shake the seeds forcefully in a container lined
with sandpaper. It may also involve mechanical
methods such as use of hot water (77 – 100 0C) or
using chemicals such as sulphuric acid.
Seed physiology…
Methods of breaking seed dormancy…
ii. Stratification:
• This involves provision of moist, aerobic conditions
during the after ripening period. In cold stratification,
dormant seed is stored at high moisture, aerated and
low temperature conditions (at 2 0C). Temperate fruits
like apples and pears are treated this way. Warm
stratification involves moist storage of temperatures
above 7 0C. This promotes germination due to
microbial decomposition of the seed cover.
Seed physiology…
Methods of breaking seed dormancy…
iii. Chipping seeds: Chipping is cutting a small
piece out of seed coat with a sharp knife.
Water can then enter the test a through the
cut. This is useful on large seeds for example,
sweet pea
Seed physiology…
Methods of breaking seed dormancy…
iv. Soaking seeds: Soaking seeds in water
overnight will soften the testa and make it
permeable to gases and water. This can be
useful for seeds such as sweet pea.
Commercial growers use diluted acids such as
sulphuric acid on some seeds.
Seed physiology…
• Methods of breaking seed dormancy…
v. Embryo culture: In some species (eg certain
types of peaches), the embryo will not be well
matured to be able to germinate when the
fruit is ripe. This is overcome by culturing the
embryo in media which provide certain types
of nutrients.
Seed physiology…
• Methods of breaking seed dormancy…
vi. Use of chemical promoters
• Some seeds contain chemicals (Chemical inhibitors)
that prevent germination. This is common in fleshy
fruit but it depends on the seed type as to what will
get rid of the inhibitor. The use of plant growth
regulators, such as gibberellins, cytokinins, ethylene,
thiourea and sodium hypochlorite can break this
type of dormancy..
Seed physiology…
Seed deterioration
Seed deterioration refers to gradual reduction
of seed ability to live.
• Seed are biological living entities, which are
regarded as a basic need for existence of
plants.
• Once seed loose the capacity to live, they are
of no value for planting purposes,
Seed physiology…
Causes of seed deterioration
• The rate of seed deterioration is highly influenced
by Field Management and handling,
environmental and biological factors.
• i. Field management prior to harvest; this poses
the greatest threat to the quality of the seed and
its longevity. Once seed deterioration begins in
the field nothing can be done to improve the
situation.
Seed physiology…
Causes of seed deterioration…
ii. Mechanical damage;
• due to post harvesting activities
• which may cause fracturing (bruises and
cracking) of essential parts of the seed, broken
seed coats permits easy entry of pathogens.
Seed physiology…
Causes of seed deterioration…
iii. Temperature; Temperature influences the
amount of moisture and also enhances the
rate of deteriorative reactions occurring in
seeds.
• High temperature hastened the rate of
biochemical processes (respiration,
enzymatic) triggering more rapid deterioration
that results into rapid losses.
Seed physiology…
Causes of seed deterioration…
iv. Moisture Content; Deteriorative reactions
occur more readily in seeds at higher moisture
content.
• Seeds stored at high moisture content lead to
increased respiration, heating, and fungal
invasion resulting in reduced seed vigor and
viability.
Seed physiology…
Causes of seed deterioration…
v. Pests, Bacteria and fungi; their respiration
(bacteria and fungi) produces energy which
may raise the seeds temperature and cause
their death.
Seed physiology…
Causes of seed deterioration…
vi. Time: time imposes the greatest unforeseen
threat, with time any living material has a
beginning and ending point.
Seed physiology…
Seed formation
• Flowering plant continues life in the form of male and
female flower structure. These floral parts are
initiated through the interaction of the plant hormonal
system with environment, including day length and
temperatures. Pollination and fertilization are
essential pre-request events for the seed formation.
• Monoecious plants have reproductive organs found in
separate flowers on the same plant.
• Dioecious plants are those in which reproductive
organs are formed in separate flowers on different
plants of the same specie
• Pollination and fertilization are pre-requisite
events for seed formation. Seed formation
takes place in female part of the flower where
the ovules inside the ovary are fertilized by
pollen grain from anthers of male part of the
flower.
• Pistil is the female flower organ which is
composed of ovary, style, and stigma. While
the male part ( stamen ) is made of anthers and
filament.
Seed physiology…
Seed formation…
• The stamen and pistil are called essential
organs because they are necessary for seed
formation. All botanical seeds at maturity
consist of 3 parts:
• Seed covering (Testa) for protection
• Supporting tissue (such as cotyledons or
endosperm), which provide nutrition
• Embryo, which carry the basic function of
propagation and multiplication.
Seed physiology…
steps on the formation of seed :
• i. The formation of reproductive organs;
stamen which is a male part of the flower and
pistil which is a female part of the flower are
formed, and the opening of the flower (to
release pollen grains) which signals the sexual
maturity of these organs;
Seed physiology…
steps on the formation of seed…
ii. Pollination; the transfer of pollen from the
stamen (anther) to the pistil (stigma) either
naturally by wind, insects, gravity or artificially
by breeders.
Seed physiology…
steps on the formation of seed…
iii. Germination of the pollen and formation of
pollen tube; The pollen grain wall splits and
pollen tube penetrates the stigma and grow
down the style to the ovule. Two male nuclei,
or sperms, move down each pollen tube to
the ovule.
Seed physiology…
steps on the formation of seed…
iv. Fertilization;
• The pollen grain and the egg cell nuclei, unite and
produce the embryo mother cell (zygote) with
the chromosome number characteristic of the
species.
• Another sperm cell fuse with two polar nuclei in
the embryo sac to form endosperm which is the
source of nutrients for the growing embryo.
Seed physiology…
steps on the formation of seed…
v. Embryogenesis;
• The embryo mother cell then undergoes
repeated divisions by mitosis to form the
embryo which is confined together with other
tissues of the seed
QUIZ!!!!!!!
QUIZ!!!!!!!!
QUIIIZ!
• Outline three parts of botanical seed at
maturity and state the function of each (6
marks) (6 minutes)
Seed physiology…
Seed development
• This is the process by which seeds are formed
from the end of fertilization to the production
of a mature seed body. After fertilization,
subsequent growth and differentiation on the
ovule occur in a series of interdependence
changes and follow a characteristic cycle for
each species.
Seed physiology…
Seed development…
• Growth of the endosperm follows the growth
of the ovule. The embryo then starts showing
a measurable increase in volume and begins
to grow rapidly after the endosperm reaches
maximum volume.
Seed physiology…
Environmental factors affecting seed
development
•Soil fertility: In general, plants that have been
fertilized with the three major elements (N, P,
and K) produce larger seeds than those which
have not been fertilized. The increase in seed
size is due to a enhanced seed development
rate during the seed filling period as a
consequence of increased nutrient availability.
Seed physiology…
Environmental factors affecting seed
development…
• Water; its deficits reduce plant metabolism
and seed development. Prolonged droughts
and reduced soil water availability cause
decreases in seed size, particularly when these
effects occur during seed filling. If water deficits
occur during flowering, its primary effect is on a
reduction in seed number.
• Light: The seasonal distribution of solar
radiation is a fundamental factor in assuring
adequate plant development. In general,
reduced light to the parent plant results in
smaller seeds.
• Temperature: High temperatures during seed
development produce smaller seeds, while
low temperatures retard seed growth. Seed
germination and vigor are also adversely
affected by exposure to low temperatures
during development. High temperatures are
considered the principal reason for the
“forced maturation” of some plants
Seed physiology…
• Seed position on the plant; The position in
the inflorescence can affect seed
development rate. For example, Soybean pods
located in lower plant branches are produced
before those located in the upper nodes and
are affected by different environmental
conditions during development and this
causes differences in seed performance.
Seed physiology…
• Maize seeds at the tip of the ear are smaller
than those at the base which has been
attributed to inadequate photosynthetic
supply. Smaller seeds are also produced from
smaller fruits or those that mature later in the
growing season or are exposed to unfavorable
environmental conditions. The usual
consequence is decreased seed germination
and vigor.
Seed physiology…
Seed maturation
• Is the morphological, biochemical,
physiological and functional changes and
differentiation that takes place from
fertilization up to the time when the seed is
ready for harvest.
Seed physiology…
Seed maturation …
• There are two types of maturity these are
physiological maturity and harvest maturity.
• The seed is considered to have reached
physiological maturity when it first attains its
maximum dry weight. After this stage there is
no more accumulation of food reserves and
the seed only loses water about 10-20%
depending upon weather conditions.
Seed physiology…
Seed maturation…
• However seed is normally not harvested at
physiological maturity, as its moisture content
is still very high.
• Most cereals reach physiological maturity at
40% moisture content
Seed physiology…
Seed maturation…
•Determination of physiological maturity.
There are three dominant physiological maturity
concepts are :
a. Physiological maturity is identified as maximum seed
dry matter accumulation
b. Physiological maturity is reached when there is no
further significant increase in seed dry weight
c. Physiological maturity occurs when seeds reach
maximum dry weight, germination and vigor
Seed physiology…
• Seed maturation…
• Harvest maturity is a stage of development at
which a plant or plant part possesses the
prerequisites for use by consumer for a
particular purpose. It is also called
horticultural maturity. Seeds attain harvest
maturity in the late stage of development.
Seed physiology…
The changes associated with seed maturity can
be summarized as follows:
i. change in the moisture content
• In the unfertilized ovule the moisture is above
80%. After fertilization, moisture increases
slightly for few days, then from that point
onwards moisture decreases rapidly until
equilibrium is established with field
environment
Seed physiology…
changes associated with seed maturity…
ii. Seed size;
• Seed size increases from the time of
fertilization and maximum size is reached at
the highest moisture content. Later the seed
size may decrease due to dryness, respiration
and activities of pathogens.
Seed physiology…
changes associated with seed maturity…
iii. Dry weight
• As seed develops, an increase in weight is very
conspicuous as a result of nutrient and water
uptake. Different parts of seed may account
for most of the weight and indicate the point
when translocation is exactly balanced by
respiration under a given environment.
Seed physiology…
changes associated with seed maturity…
iv. Seed viability and vigour;
• Many seeds can germinate few days following
anthesis, i.e. immature seeds can germinate
but vigour increases until it reaches its
maximum at physiological maturity.
Seed physiology…
changes associated with seed maturity
v. Chemical and other changes.
• Carbohydrate content increases rapidly
especially in endosperm of monocots with
development.
• Sucrose and reducing sugar, initially high,
decrease rapidly as starch content rises.
• Protein increases for a certain period of time
and remains constant.
Seed physiology…
Seed germination
• Germination is the resumption of growth of
the seed (change from arrested development
to active growth).
• It is the emergency and development from
the seed embryo of those essential structures
that make a seedling.
Seed physiology…
• Seed germination…
Seed physiology…
Conditions for germination.
• Germination requirements are;
i) Water: the seed must be supplied with
adequate moisture to dilute its contents eg.
Stored food and to soften the seed coat
(testa).
Seed physiology…
Conditions for germination…
ii) Oxygen: sufficient oxygen is required by the
cells for respiration. During respiration the
food substances such as glucose are broken
down to release energy needed for cell
division. The recommendation that seedbed
should be loose and the seed planted at a
shallow depth aims at adequate supply of
oxygen.
Seed physiology…
Conditions for germination…
iii) Temperature: The optimum temperature for most
seeds is between 30 oC -35 oC. At low temperature the
enzymes become inactive while at high temperature
enzymes will be denatured, hence seed will not
germinate. Therefore, temperature requirements
varies with plant species. Cold resistant plants have a
minimum temperature of 0 oC – 5 oC and optimal
temperature of about 28 oC – 30 oC. cold sensitive
plants have their optimum in the range of 30 oC -35 oC.
Seed physiology…
Conditions for germination…
iv) Light: especially red and far red light affects
germination of some seeds. In many seeds
which require light, gibberellins can replace
light and stimulate germination.
Seed physiology…
Phases of seed germination
The germination process is divided into three
phases;
• imbibitions phase
• saturation phase (lag period)
• growth phase
Seed physiology…
Phases of seed germination…
i. Imbibitions (phase I)
• The seed, first imbibe (absorb) water, the
tissues swell and the seed coat becomes soft
and elastic. When the seeds imbibe water,
various substances gasses, salts sugars are
driven out and their uptake increases. Oxygen
is necessary for the breakdown of food
reserve.
Seed physiology…
Phases of seed germination…
ii.Saturation (phase II)
• During this phase, preparation for germination
takes place whereby mobilization of food
reserve starts after the onset of germination.
The reserve food is mainly meant for the
growth of the seedling.
Seed physiology…
Phases of seed germination…
iii. Growth (phase III)
• The swelling of the seed by imbibitions causes
the testa to split open easing growth. Depending
on the species, visible germination can occur
several hours or days after imbibitions phase. The
radical grows downward between the soil
particles while the plumule emerges and grows
upwards the soil surface.
Seed physiology…
• Seed germination test
• Germination test shall be conducted with a
pure seed fraction.
• A minimum of 400 seeds are required in 4
replicates of 100 seeds each or 8 replicates of
50 seeds each or 16 replicates of 25 seeds
each depending on the size of seed and size
of the containers of substrate.
Seed physiology…
GERMINATION PERCENTAGE
• The germination percentage is the proportion of
seeds that germinate from all seeds subject to
the right conditions for growth.
• It is an estimate of the viability of a population of
seeds.
Mathematically;
• Germination %(GP) = germinated seed x 100%
Total number of seeds
Seed physiology…
Procedure for germination test of a seed;
• Obtain a Petri dish and a filter paper
• Select a seed source and record the name
• Count out 50- 400 seeds(fewer if large seeds)
and soak them in water for about an hour
Seed physiology…
Procedure for germination test of a seed…
• Spread the seeds on 2-3 layer of filter paper in
the Petri dish
• Moisten the paper with enough water(about
10 mls) so just barely begin to float
• Put on the lid cover the seeds with wet filter
paper and place the dish in the dark (safe
place)
Seed physiology…
Procedure for germination test of a seed…
• Record the number of seeds that germinate
daily for 5-7 days(according to germination
duration of the seed in question)
• Use the formula above to calculate the
germination percentage of the seeds
Seed physiology…
EVALUATION OF THE SEEDLINGS;
• At the end of the test, evaluation will take
place in order to classify the germinated
seedlings and non germinated seeds.
• This will be done as follows;
Seed physiology…
Evaluation of the seedlings…
i) Normal seedlings
• Are those seedlings that show potential for
continued development when the grown in
good quality soil(media/substrate) under
favourable conditions of moisture, temperature
and light.
• The seedlings have well developed radicals and
plumules.
Seed physiology…
• Evaluation of the seedlings…
ii) Abnormal seedling
• Are those seedlings, which fail to show the
potential to develop into a normal plant,
when grown in good quality soil
(media/substrate) under favourable
conditions of moisture, temperature and light.
Seed physiology…
• Evaluation of the seedlings…
iii) Dead seeds
• These are seeds, which at the end of test
period are neither fresh nor hard, not showing
any sign of germination.
Seed physiology…
• Evaluation of the seedlings…
iv) Fresh and ungerminated
• These are dormant seeds, which even after
soaking and at the end of the test remain firm.
• These are not dead seeds, but will germinate
if dormancy is overcome naturally or by using
artificial methods
Seed physiology…
• Roles/importance of seed germination
percentage;
• To asses seed quality and viability attributes of
the seed lots which have to be offered for
sale. It determines if seed meets established
quality standards or labeling specifications.
• It provides a basis for price and prevents
consumer discrimination among lots in the
market.
Seed physiology…
• Roles/importance of seed germination
percentage…
• To predict the performance of the seed and seedling
in the field. Germination rate of a particular seed lot
is a key indicator as to how that seed will perform in
the field.
• The information is important for calculating optimal
seedling rates as well as helping to determine
whether a particular seed lot has the potential to
produce a good crop.
Seed physiology…
Seed purity test…
• Pure seed is a seed free from admixtures of all
kinds such as weed seeds, seeds from other
crops and inert matters.
Seed physiology…
Procedure for seed purity test:
• i. Take and mix a representative sample of
seeds from the top, middle and bottom of the
bag or container.
• ii. Weigh a convenient amount of seeds from
the sample by using a weighing scale.
• iii. Sort to separate the seeds from admixtures
Seed physiology…
Procedure for seed purity test…
• iv. Weigh the admixtures. Where the
admixtures are too small to be weighed on
available weighing scales, the pure seed
should be weighed and weight of admixtures
obtained by difference.
Seed physiology…
Procedure for seed purity test…
• v. Calculate Percentage pure seed using the
formula below;
Back cross…
• For example: Consider a hypothetical cross in
which genes for stem rust resistance (RR) are
to be added to an adapted high yielding
variety A of wheat.
INTRODUCTION TO PLANT
BREEDING…
The Procedure for back crossing:
i. The donour (resistant) variety is crossed to the
recurrent (adapted) variety A. The F1
generation from this cross will be segregating
for the rust resistance (RR: rr)
INTRODUCTION TO PLANT
BREEDING…
The Procedure for back crossing…
ii. The Rr plants may be identified from the rr
plants by inoculating the seedling plants with
the rust pathogen and observing whether the
plants exhibit resistant or susceptible disease
reactions.
INTRODUCTION TO PLANT
BREEDING…
The Procedure for back crossing…
iii. Back cross the resistant plants (Rr) to variety
A in the second and succeeding back cross
generations.
iv. After the final back cross the resistant (Rr)
plants are selfed one generation to obtain
homozygous RR and heterozygous Rr resistant
plants.
INTRODUCTION TO PLANT
BREEDING…
The Procedure for back crossing…
v. Progeny test of the resistant (RR and Rr)
plants are grown to identify the homozygous
(RR) from the heterozygous (Rr) plants, so that
lines homozygous for resistance may be
established and released as a new variety.
INTRODUCTION TO PLANT
BREEDING…
COMPOSITE (OPEN POLLINATED) FORMATION
• Composite as the name implies, are groups of
open pollinated varieties, inbred or other
germplasm units that are pooled in same
manner.
• Original entries with composite are not
maintained for reconstituting the composite
and new entries may be inserted.
INTRODUCTION TO PLANT
BREEDING…
The Procedure for composite formation:
i. A germplasm pool adapted to various climatic
conditions is formed and their families are
crossed in isolation and evaluated to obtain
recombination among the parental families.
• Each families is planted separately so that it
will serve as a seed plant
INTRODUCTION TO PLANT
BREEDING…
The Procedure for composite formation…
ii. A mixture of seed from all parents is used as
the pollen parent
iii. From each family, superior ears are chosen to
provide seed for the next cycle.
• Families may be dropped, or new families
added with each cycle
INTRODUCTION TO PLANT
BREEDING…
The Procedure for composite formation…
iv. Seeds from superior families are tested in
yield trials at several locations
v. The upper 10 % o families with the best
performance in the yield trials will be tested in
yield trials at multilocational sites.
INTRODUCTION TO PLANT
BREEDING…
The Procedure for composite formation…
vi. Those with good performance are released as
cultivars.
• Examples of open pollinated varieties released
in Tanzania are Staha, Kito, TMVT2, Kilima ST,
Kito ST, Katumani ST
• Varieties with ST are tolerance to maize
streak virus disease
SEED PRODUCTION SYSTEM
• Seed production system represents
involvement and interconnection among
different organizations, institutions and
individuals associated with the development
of new varieties and producing, testing,
processing, storage, certifying and marketing
seed to farmers.
• SEED ACT REGULATES SEED INDUSTRY IN
TANZANIA(No 18 of 2003)
• An act to make provision for the control and
regulation of the standards for agricultural
seeds and for matters incidental thereto and
therewith. The purpose is to prescribe the
plant varieties and standard of the seeds for
importation, exportation, production,
processing and distribution.
SEED PRODUCTION…
SEED PRODUCTION SYSTEMS
Three seed production systems do exist in
Tanzania:
1.Formal seed production system
2.Semi formal seed production system(Quality
declared seed)
2. Informal seed production system
SEED PRODUCTION…
1. FORMAL SEED PRODUCTION SECTOR OR
SYSTEM
• This is a framework of institutions linked
together by their involvement in or influence
on the germplasm manipulation (breeding),
multiplication, processing, marketing and
quality control of seeds. , Its is characterized
with well coordinated components of a seed
industry to ensure a regular supply of large
amount of uniformly high quality of seed to
farmers.
SEED PRODUCTION…
• The National Seed Committee
established/approved in 1994 whereas the
Permanent Secretary of the Ministry of
Agriculture is the chairperson.
• The National seed policy of 1994 seeks to
ensure a collaborative effort of both
government and private sector to achieve
national seed self – sufficiency.
SEED PRODUCTION…
• Characteristics of formal seed sector.
i. Quality assurance.
ii. Planned seed production.
iii.Defined varieties from formal breeders.
iv.Mechanical processing of seeds.
v. Seed sold in labeled package.
SEED PRODUCTION..
• In Tanzania , the formal seed production is
now undertaken by,
i. Government Foundation Seed Farms-Official
seed production.
ii. Government Parastatal
Organization(TANSEED) semi official seed
production.
iii.Private Seed Companies-Private Seed
Production.
SEED PRODUCTION…
I. COMPONENTS OF THE COMPLETE FORMAL
SEED SECTOR/SYSTEM;
A. Plant breeding, variety assessment and
maintenance (Agricultural research);
• In Tanzania, both private and public sectors
have viable plant improvement programme
which are vital for the sustainability of a seed
industry.
SEED PRODUCTION…
I. COMPONENTS OF THE COMPLETE FORMAL SEED
SECTOR/SYSTEM;
A. Plant breeding, variety assessment and maintenance
(Agricultural research)…..
The main tasks of researchers are;
• The continuous development of improved plant varieties
to enhance productivity in various agro-ecological zones
of a country
• Evaluation of new promising varieties for Value for
Cultivation and use (VCU) and Distinctness Uniformity
and Stability (DUS).
• Tanzania Official Seed Certification Institute (TOSCI)
undertakes DUS and VCU tests.
SEED PRODUCTION…
I. COMPONENTS OF THE COMPLETE FORMAL
SEED SECTOR/SYSTEM;
A. Plant breeding, variety assessment and
maintenance (Agricultural research)…
The main tasks of researchers …
• Maintenance of their own varieties
• For locally variety development, evaluation,
release and maintenance of eligible varieties as
Pre-basic seed is done by the government crop
research stations, private seed companies and
Sokoine University of Agriculture (SUA)
SEED PRODUCTION…
• In Tanzania, the National Agricultural
Research Service in crop has been divided into
eight (08) main centres namely:
• TARI ILONGA, Kilosa, Morogoro region. The
leading commodity crops researched on are
low altitude maize, sunflower,
sorghum/millet, grain legumes (except beans).
It also coordinates post harvest management.
SEED PRODUCTION…
• TARI TUMBI, Tabora region. The leading
commodity crops researched on are oil palm
and agro forestry.
• TARI UKIRIGURU, Mwanza region. The leading
commodity crop researched on is cotton, and
root and tuber crops (except Irish potatoes).
• TARI SELIAN, Arusha region. The leading
commodity crops researched on are wheat,
barley and medium altitude maize.
SEED PRODUCTION…
• TARI UYOLE, Mbeya region. The leading
commodity crops researched on are beans,
high altitude maize, irish potatoes and
pyrethrum. It also coordinates Agro
mechanization.
• TARI NALIENDELE, Mtwara region. The
leading commodity crops researched on are
cashew and oil crops (except sunflower).
SEED PRODUCTION…
• TARI MAKUTUPORA, Dodoma region. The
leading commodity crop researched on is
Viticulture.
• TARI MLINGANO, in Tanga region. The leading
commodity crops researched are Sisal, Soils
and water management, it also coordinates
soil laboratory services in the country.
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)
• This is done by seed farms of the government
and private seed companies.
• Initial increase of pre basic and basic seeds to
substantial quantities for further bulk production
is a necessary component to help attain required
see supply for the farming community.
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)….
• Usually, multiplication of a variety is done
strictly under the supervision of the
originating breeder or institution basically to
safeguard variety propriety rights.
• It may be planned to cover several seasons
with provision of good storage
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)….
• This provision alleviates the risks of poor
weather, cut down production costs and
minimize isolation constrains.
• This pedigree seed is subject to certification
procedures as it forms the heart of seed
quality control.
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)….
• The Ministry of Agriculture has five (05)
foundation seed farms, all allocated strategically
to serve the main agro-ecological zones namely:
• Arusha foundation seed farm, located at
Ngaramtoni, Arusha, multiplies crops suited from
mid-altitude to high lands like maize, wheat and
beans.
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)….
Foundation seed farms….
• Dabaga foundation seed farm, located at
Dabaga, Iringa, produced seeds for mid-
altitude to high lands like maize, wheat, beans
and soy beans.
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)….
Foundation seed farms….
• Msimba foundation seed farm, located at
Kilosa, Morogoro, increases seeds for lowland
and mid-altitudes like maize, beans, cow peas,
green grams, sorghum, sunflower and millet.
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)….
Foundation seed farms….
• Mwele foundation seed farm, located at
Maramba, Muheza, Tanga, produces seeds for
low land and mid-altitudes like maize,
sorghum, millets,ground nuts, sesame and
sunflower.
SEED PRODUCTION…
B. Initial seed multiplication (multiplication of
released varieties from Pre – basic to
Foundation/ basic seed)….
Foundation seed farms….
• Kilangali foundation seed farm, located near
Kilosa, Morogoro, the only farm which
multiplies paddy/rice.
SEED PRODUCTION…
C. Bulk multiplication of seed from basic to the
grades Certified 1(C1) and Certified 2 (C2):
• The private seed companies undertake this
process through their contract
growers.Government seed farms also produce
C1 and C2.
SEED PRODUCTION…
D. Seed quality control:
• Seed quality control is an essential component
for protection of seed consumers from
possible exploitation by unscrupulous seed
merchants.
• Control involves monitoring of seed quality
throughout the various stages of production,
processing and marketing (Field inspection,
seed testing, pre and post control)
SEED PRODUCTION…
D. Seed quality control…
• Seed quality control services are backed up by
a suitable legislation, the seed act of 2003, in
order to ensure that seed reaching the
farmers is of the prescribed standards.
• A continuous supply of good quality seed of
improved varieties provides an excellent basis
towards improving crops production in the
interest of achieving food security.
SEED PRODUCTION…
D. Seed quality control…
• Seed quality control can be performed by seed
enterprises through licensing scheme as their
personnel become competent after training.
• A comprehensive regulatory seed quality control
scheme where legislation is reinforced requires
well equipped laboratories for seed sampling and
inspection, seed testing, labelling and quarantine
operations for imported seeds.
SEED PRODUCTION…
D. Seed quality control…
• The TOSCI is the only body allowed by the law
(i.e. The Seeds Act of 2003) to undertake
certification activities in the country.
• It has its headquarters in Morogoro with
branches in Tengeru, Arusha region, and Njombe,
Njombe region.
• The institute has a training programme after
which seed companies and individuals may be
licensed to undertake quality control of their own
seeds.
SEED PRODUCTION…
E. Processing and storage (seed conditioning)
• The private seed companies as well as
government seed farms undertakes
processing and storage.
SEED PRODUCTION…
F. Marketing
• Seed marketing and distribution are usually
performed by the seed companies through a
network of selling points or seed stockists.
SEED PRODUCTION…
G.SEED EXTENSION SERVICES:
• Seed extension is done by Government or
private seed companies to ensure that
farmers obtain maximum benefits from high
quality certified seeds which are suitable for
their growing conditions.
SEED PRODUCTION…
G.SEED EXTENSION SERVICES…
The main objectives of seed extension services
are:
• i. To inform farmers on the existence and
advantages of certified seed;
• This is the first process of seed promotion.
Farming community must be informed on new
findings which will help them to increase their
yields.
SEED PRODUCTION…
G.SEED EXTENSION SERVICES…
The main objectives of seed extension services…
ii. To convince farmers to use certified seed on
a large scale;
• An extension programme will have achieved
its objectives when the majority of farmers
accept the change and adopt the use of
improved seeds along with the recommended
techniques.
SEED PRODUCTION…
G.SEED EXTENSION SERVICES…
The main objectives of seed extension services…
iii. To inform farmers of the ways and means for
the acquisition of certified seed;
• The extension workers must be aware of all the
possibilities of obtaining certified seeds and
advise farmers accordingly. Various players in the
seed programme include seed growers, seed
processors, seed merchants and farmers etc.
SEED PRODUCTION…
G.SEED EXTENSION SERVICES…
The main objectives of seed extension services…
iv. To train farmers on recommended production
techniques for the cultivation of improved seed;
• The introduction of seed of improved varieties
necessitates the application of new production
techniques, such as sowing method, fertilizer
application, irrigation, pest treatment, harvesting
and various measures which have to be taken to
prevent seed from being mixed with other
varieties.
SEED PRODUCTION…
G.SEED EXTENSION SERVICES…
The main objectives of seed extension services…
v. To establish an effective communication
between farmers using improved seed and
plant breeders;
• This plays an important role in providing seed
scientists with information from the field
which could be of great value to their research
programme.
SEED PRODUCTION…
• THE FORMAL SEED SECTOR - ORGANIZATION
STRUCTURE:
• (DIAGRAM)
SEED GRADES: THROUGH THE PRODUCTION STAGES:
30cm
Sow one seed per hill at Sow 7 seeds per hill at
1.8 cm intervals 12.5 cm intervals 379
SEED PRODUCTION…
• MAIZE SEED PRODUCTION- (Zea mays L.)
• Hybrid maize production;
• Hybrid is a product of a cross between
genetically unlike parents. The main feature of
a hybrid variety is uniformity or little
variability and heterosis.
SEED PRODUCTION…
• There are four kinds of hybrid maize:
• i. single cross;
• Formed by hybridizing two inbred lines e.g. A
xF
• ii. Three way cross;
• One of the parents is single cross and the
second is an inbred line e.g. (A x F) x G
SEED PRODUCTION…
iii. Double cross;
• Each of the component parents is a single
cross e.g. (A x F) x (D x G)
iv. Top cross;
• One of the component parent is a pure
composite Open pollinated e.g. (A x F) x
composite Ec 573
SEED PRODUCTION…
Site selection.
• Soil: Maize grows well on well drained soils
which have a good supply of nutrients at a PH
range of 4.5 – 7.0. It cannot tolerate water
logging.
SEED PRODUCTION…
• Isolation distances: the crop is cross
pollinated and the recommended isolation
distances are as shown in the table below.
Isolation by time can also be practiced by
establishing crops at different sowing dates,
such that flowering differs by 28 – 30 days.
SEED PRODUCTION…
• Cropping history: the maize seed crop shall
not be grown on land on which the previous
crop was maize unless it was of the same
variety. This requirement is necessary in order
to avoid the possibility of volunteer plants
arising from seed of the previous crop.
SEED PRODUCTION…
• Specific techniques for hybrid maize seed lot
establishment:
• Female and male rows are sown at the ratio of
4:2 and/or 6:2 depending on the efficiency of
male to produce pollens.
SEED PRODUCTION…
• Marking for identification of each of the
parent rows in the field/plot is very essential
to avoid confusion during other
managements. Male rows can be marked by
pegs during planting.
SEED PRODUCTION…
• Synchronization of parental rows is that the
male parent may flower slightly late to allow
for enough period for the female parental
rows to stabilize in silking.
SEED PRODUCTION…
• If there is a reasonable difference in period of
flowering between the two parental rows,
sowing of one of the parents can be done at
different periods to respectively ensure that
flowering occur precisely at the same period.
SEED PRODUCTION…
• Seed rate for female ranging from 16 to 18
Kg/Ha and male range from 6 to 7 Kg/Ha at
the spacing of 75cm – 90 cm by 30 cm.
SEED PRODUCTION…
• Detasselling (removal of tassels) should be
done of female maize rows before the onset
of pollen shed and that only female rows will
be harvested as hybrid seeds while male rows
will be used as food crop.
SEED PRODUCTION…
• Harvesting
• Sign of maturity; the seed shows a dark spot
on the side of embryo.
• It is advised not to harvest too early or too
late.
SEED PRODUCTION…
• If it is harvested too early; the crop may be
vulnerable to shrinkage of kernels, mouldiness
and rots.
• Too late harvesting expose the crop to rots
and seed sprouting in the field if showers
prevail.
• Care must be taken to avoid mixing of seed
and the male parent material.
SEED PRODUCTION…
• The male parent should be thoroughly
removed from the field before starting to
harvest the seed parent.
QUIZ!!!!!!
QUIZ!!!!!
• List four (4) kinds/types of hybrid maize (4
minutes)
SEED PRODUCTION…
• STEPS OF ESTABLISHING COMMON BEAN
SEED LOT.
• Incorporate well matured FYM, compost (5
tones/Ha) and/or DAP (150 kg/Ha) at sowing
time because beans are responsive to residual
fertilizers.
SEED PRODUCTION…
• Where bean seed production is dependent of
rainfall, planting dates should be adjusted to
allow harvesting to take place after the main
rains are over.
• Whereas, bean seed production depends on
irrigation, a dry season crop may be grown if
irrigation water is available.
SEED PRODUCTION…
• Seed rate range between 30 – 45 kg/Ha and
dibbling method is advocated whereas the
hole is opened and single seed placed and
firmly covered.
• Spacing for common bean as seed crop should
be 50 cm row space and 10 cm intra-row
spacing to make a plant population of 200 000
plants/Ha.
SEED PRODUCTION…
SUPPLEMENTARY IRRIGATION:
• Irrigation is application of water to the soil to
ensure adequate moisture supply to meet the
seed crop needs.
• Supplementary irrigation is addition of water
to the soil at a dry spell within rainy season for
the supply moisture to meet the seed crop
needs.
SEED PRODUCTION…
• The objective is to prevent moisture from
becoming a limited factor of plant growth and
development.
• Irrigate when rainfall is not sufficient and/or
poorly distributed.
SEED PRODUCTION…
• ROUGING:
• Rouging is the process of removing unwanted
plants from the seed production fields/lots, as
well as plants infected with seed born
diseases.
SEED PRODUCTION…
Undesirable plants include;
• off-type plants,
• other plant varieties,
• plant of other crop species and diseased
plants.
SEED PRODUCTION…
Off-types = plants or seeds which do not
conform to the characteristics of the variety.
• Any plants that shows unusual growth or
morphological characteristics such as different
colour, days to maturity, is regarded as an off
type and must be removed.
SEED PRODUCTION…
• A good indication to show that rouging has
been completed successfully is that, the
remaining seed crop is uniform, pure and
therefore conforms to the prescribed
characteristics of variety under consideration.
SEED PRODUCTION…
The following characters should be born in
mind for the rouging to take place:
• General plant appearance.
• Flower colour and shape.
• Plant height
• Maturity period
• Colour of stem and leaves
SEED PRODUCTION…
ASSURANCE OF WHAT TO BE ROUGED:
• To know the morphological characteristics of
the seed crop in question.
• To know the morphological characteristics of
the variety in question.
SEED PRODUCTION…
• Abide on the Distinctiveness, Uniformity and
Similarity (DUS) as provided by certification
agency.
• The rule of thumb is ''DOUBTS OUT'' any plant
with doubtful description whether definite or
doubtful must be rouged out.
SEED PRODUCTION…
FIELD SEED CROP INSPECTION:
• Is inspection of seed field by seed field
inspector to check for none conforming
factors.
• The inspector will assess each factor to
confirm whether the seed crop fulfils the
certification requirements.
SEED PRODUCTION…
• For self or open pollinated two inspections are
required first before flowering and second
before harvesting.Hybrids requires a minimum
of four inspections; one before flower
initiation, second at flowering, third at grain
filling and fourth at maturity before harvesting
SEED PRODUCTION…
The purposes of pre-harvest inspection are:
• Make a final check on rouging
• Confirm if male lines in case of a hybrid seed
field have been removed.
• Authorise harvest if applicable
SEED PRODUCTION…
HARVESTING AND POST HARVEST
TECHNIQUES OF SEED CROPS:
Avoid seed mixture during harvesting and
storage
harvesting seeds earlier than the crop to
escape seed mixtures by;
• using new containers,
• Using new gunny bags with a proper labelling
SEED PRODUCTION…
Note;
• The time of harvesting is determined by
proper stage of ripeness.
• Harvesting too early or too late reduces the
crop yield ,
• by harvesting too late losses may occur
through shattering lodging or other pre
harvest losses.
SEED PRODUCTION…
• Manual harvesting or stoking should start
between the end of yellowing ripeness and
full ripeness.
• When combine harvester is used and the
weather is good harvesting should start after
full ripeness or dead ripeness so that the
moisture content of the grain is 10-14
percent.
SEED PRODUCTION…
• In case of grain legume, the harvesting
process should start at full ripening or dead
ripening.
• Losses due to pod shattering can be
minimized by harvesting early in the day.
SEED PRODUCTION…
• Methods of harvesting
– Hand harvesting- The product can be picked by
hand straight from the plant. This method is
common to most legumes and cereals
SEED PRODUCTION…
• Stoking
• In some crops like maize or sesame the crops
sometimes harvested by cutting the stock off
the ground and then stoked for a period of
time to allow the harvested crops to continue
drying before removal of ear or threshing
SEED PRODUCTION…
– Machine harvesting – corn pickers can be used to
remove cobs from the plants
• Combine harvesters – used for closed drilled
crops and for crops planted in rows, provided
no any selection of cobs, head or spike to be
done after harvesting.
SEED PRODUCTION…
• Processing follows the same steps, but
chemicals for preservation and condition of
store are specific.
SEED PRODUCTION…
• The following are general steps (seed
processing) to be followed after harvesting a
seed crop:
SEED PRODUCTION…
• SEED DRYING – harvested seed lots must not
have too high moisture content that should be
well dried (naturally or artificially) before
performing next step.
• Natural drying is the process whereby the
threshed or unthreshed crop is dried by sun
and wind when the relative humidity is low.
SEED PRODUCTION…
• Disadvantages/demerits of natural drying:
• Time consuming; the spreading of the crop on
the drying yard and collection of the crop after
drying requires a lot of time and it is tedious
since drying of some crops can last for 2 -3
days.
SEED PRODUCTION…
• Too fast drying
• Since the temperature is not controlled, there
is a risk of fast drying of the seed. Fast drying
may lead to cracking of the seed and reduce
its quality.
SEED PRODUCTION…
• It pollute the seed
• Since the seeds are dried on the open area,
the seed can easily be polluted with dust and
dirt as well as insect pest infestation. This may
also cause the seed to deteriorate.
SEED PRODUCTION…
• THRESHING – is the process of separating
seeds from other part of the plant (stocks or
straw). The action may be as trail beating or
rolling of plant or by thresher (threshing
machines)
SEED PRODUCTION…
• SEED CLEANING – removal of all chaffs, parts
of the plants and dirt’s simply by winnowing
manually or by machines.
• SAMPLING – random picking of the
representative of the seed lot are combined
and submitted for purity analysis.
SEED PRODUCTION…
• SEED TREATMENT:
• Before disposal of the clean seed for storage
or use, should be treated (seed dressing)
against insect pests and pathogens.
SEED PRODUCTION…
Criteria/factors that the ideal seed treatment
method should adhered to
i. Effectiveness
• The seed treatment method to be used should
be very effective against seed-borne
pathogens that can destroy the stored seeds.
SEED PRODUCTION…
• ii. Relatively non toxic
• The method should be relatively nontoxic to
animals and plants, even if misused, therefore
if chemical method is to be used the
concentration of active ingredient should
affect only targeted pathogens.
SEED PRODUCTION…
• iii. Longevity
• The proper method should be stable for
relatively long period of time during seed
storage, before pathogen to reappear
SEED PRODUCTION…
• iv. Easy to use/apply
• Proper method should be user friend to allow
normal farmers to use without a need of
expertise.
SEED PRODUCTION…
• v. Environmental friend
• The method used should be acceptable
according to environmental safety standards
and have minimum residual effects to soil and
water bodies.
SEED PRODUCTION…
• vi. Economical
• The method used for seed treatment should
be economic to minimize production cost.
SEED PRODUCTION…
• PACKAGING- the treated seed is therefore
packed in new clean bags of good strong
materials. May be packed in different size
according to customer market demands,
normally is done in 02 kg, 05 kg, 10 kg, 20 kg
and 50 kg.
SEED PRODUCTION…
• LABELING AND SEALING – seed of all pedigree
classes must bear the official label attached to
each bag which shall show lot number, kind of
crop, name of variety, class of seed,
germination percent, purity and date of test.
SEED PRODUCTION…
• SEED STORAGE: - These are activities starts
from after harvesting to sowing, the seed is
kept under storage. The storage period can be
for a relatively short or long period of time,
and at conditions which cause minimum
reduction of viability.
SEED PRODUCTION…
Reasons for seed storage:
• May be uneconomical to multiply.
• Not always possible to estimate seed yields.
• Good seed stock are valuable may be difficult
or costly to multiply.
• Demands for seed may fluctuate
SEED PRODUCTION…
FACTORS WHICH REDUCE SEED LONGEVITY IN
THE STORE:
• Mechanical damages
• Insect pest infestation
• Diseases (pre- and post- harvest).
• Immature or under ripening of seed at harvest
• High moisture content of seed than normal
SEED PRODUCTION…
• TYPES OF STORAGE AND FACILITIES: The total
seed storage period includes the following
sequential order:
• Bulk storage – this is storage between
threshing, drying and cleaning, bins are used
as storage facilities.
SEED PRODUCTION…
• Holding storage – this is storage between
different steps in processing and from
processing to packaging, bins and containers
are used.
SEED PRODUCTION…
• Package seed storage – this is storage
between package and distribution, ordinary
packets of sizes 10 kg, 20 kg and 50 kg are
used.
SEED PRODUCTION…
• Market place storage – storage between
distribution and sales, packets arranged in
stack.
• Farm storage – storage between purchase
and planting, farm storage is usually
temporary and is just before planting or after
harvest.
SEED PRODUCTION…
STORAGE PERIOD:
• Short term storage – from harvesting to the
next planting season, range between 01 to 09
months, condition for safe storage: 30 0C and
RH 50%, MC 12% for cereals and 08% for oil
seeds and legumes or 20 0C RH 60%, MC 13%
for cereals and 09.5% for oil seeds and
legumes.
SEED PRODUCTION…
• Medium term storage – storage duration
range between 18 to 24 months, condition for
safe storage: 30 0C and RH 45%, MC 10% for
cereals and 02.5% for oil seeds and legumes
or 20 0C RH 50%, MC 12% for cereals and 08%
for oil seeds and legumes and/or 10 0C and RH
60%, MC 12% for cereals and 09% for oil seeds
and legumes.
SEED PRODUCTION…
• Long term storage – storage duration range
between 03 to 10 years, condition for safe
storage: 20 0C and RH 45%, MC 02.5% to 05%
for breeders seeds, ornamentals and
vegetable seeds.
SEED PRODUCTION…
PEST MANAGEMENT DURING STORAGE:
• Rodents: Apply integrated Storage rodent
management as follows:
• Rat proofing in the store (kihenge) by raising
the floor to the above ground level at 1.0 m
and fixing of aluminum sheet of V-shape on
posts.
SEED PRODUCTION…
• Repelling rats by chemical repellents or
change of environment or use of ultra sound
machine.
• Trapping of rats in small population in the
store or warehouse and/or godown.
SEED PRODUCTION…
• Habitat modification by removing all
harbourage and alternative food and water.
• Use of rodenticides either acute or chronic
when the population of rats and mice
surpasses economic threshold.
SEED PRODUCTION…
• Insects: Apply integrated storage insect pest
management as follows:
• Treat the seed with recommended storage
insecticides.
• Disinfect the packaging materials with
recommended disinfectant.
SEED PRODUCTION…
• Disinfect the store house/ware house/godown
with recommended disinfectant.
• Arrange the well packed seeds in a pallet,
while dusting on bags with the chemical used
to treat the seed lots.
SEED PRODUCTION…
• Inspect the store once a month to check for
any activities of insect pests
• Abide on rules of the store that first in the
store, should be first out of store.
SEED PRODUCTION…
• Fungus: Apply integrated storage fungal
disease management as follows:
• Disinfect the seeds by using heat (thermal
treatment) or by treating the seeds with
fungicides.
SEED PRODUCTION…
• Check the seed in store regularly and use
damage thresholds for re-dressing.
• Plan and use fungicides at proper time, place,
chemical in a proper dosage
VEGETATIVE PROPAGATION BY
BUDDING
Budding is grafting with a scion possessing only
one bud or eye of buds.
• It is used in citrus fruits to bud good varieties
of fruit onto vigorous and resistant stock
plants
VEGETATIVE PROPAGATION BY
BUDDING…
Conditions for the successful grafting;
• The scion and the root stock must be
compatible, graft compatibility means that the
scion and the rootstock are capable of joining,
that is they should be related eg. Cultivar of
the same species or species of the same
genus. Sweet orange can be grafted onto
lemon
VEGETATIVE PROPAGATION BY
BUDDING…
Conditions for the successful grafting…
• The grafting technique must be one that is
successful with the species being grafted.
Some species are more likely to graft by one
technique than another and therefore the
recommended grafting technique for a
particular species should be used.
VEGETATIVE PROPAGATION BY
BUDDING…
Conditions for the successful grafting…
• The grafting operation must be performed
well and at the right time of the year, most
grafts are performed slightly before or during
a period of active growth for rapid healing.
VEGETATIVE PROPAGATION BY
BUDDING…
Conditions for the successful grafting…
• The environment must be suitable to
encourage the graft to heal e.g. high humidity
and warm temperatures between 13 oC and
32 oC are preferred.
VEGETATIVE PROPAGATION BY
BUDDING…
Conditions for the successful grafting…
• The scion must be of the previous growing
season and it must have dormant bud or eyes
• The scion and the root stock should have the
same size ( pencil size ) to ensure the good
contact between their vascular cambium
VEGETATIVE PROPAGATION BY
BUDDING…
The main two methods of budding;
• T- budding
• Inverted T- budding
VEGETATIVE PROPAGATION BY
BUDDING…
Procedure for inverted T- budding;
• Select young vertical shoots from high yielding
and disease free orange trees
• Removes all shoots and thorns from the stem
on the lower part of the root stock seedling
• a vertical cut about 2-3 cm long is made in the
stock
• A 2cm long horizontal cut is made at the base of
the vertical cut
VEGETATIVE PROPAGATION BY
BUDDING…
Procedure for inverted T- budding…
• beginning 2 cm above a bud a slicing downward
cut is made 1 cm below the bud, a horizontal
cut is made at the base to remove the bud
• The bud is inserted by pushing it upwards under
the bark flaps on the stock
• The two horizontal cuts are matched and the
bud union is tied with a water proof material
VEGETATIVE PROPAGATION BY
BUDDING…
Procedure for normal T- budding;
• Select young vertical shoots from high yielding
and disease free orange trees
• Removes all shoots and thorns from the stem
on the lower part of the root stock seedling
• a vertical cut about 2 cm long is made in the
stock
• A 2cm long horizontal cut is made at the top of
the vertical cut
VEGETATIVE PROPAGATION BY
BUDDING…
Procedure for normal T- budding…
• beginning 2 cm below a bud a slicing upward
cut is made 1 cm below the bud, a horizontal
cut is made at the top to remove the bud
• The bud is inserted by pushing it downwards
under the bark flaps on the stock
• The two horizontal cuts are matched and the
bud union is tied with a water proof material
VEGETATIVE PROPAGATION BY
BUDDING…
CHECKING AND ATTENDING THE BUDDING;
• Continue irrigating and weeding
• After two weeks examine the bud if it still
green, then union has taken place
• Remove the wrapping material a bit on upper
and lower parts of the bud
VEGETATIVE PROPAGATION BY
BUDDING…
CHECKING AND ATTENDING THE BUDDING…
• Cut the root stock 10 cm above the budding
point and remove any suckers which develop
on the root stock below or above the budding
point
• carryout pruning and other normal nursery
management practices awaiting for
transplanting