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PROTEOMICS
PROTEOMICS
Introduction
y Proteomics is the large-scale study of proteins, y y y y
particularly their structures and function. Proteomics is the systematic study of all of the proteins in a cell, tissue, or organism. The term proteomics was coined in 1994 by Marc Wilkins. The study of the Proteome. The proteome is the entire set of proteome expressed by a genome, cell, tissue or organism.
Proteomics classification
y Expression y Cell map or Interaction y Functional y Structural
abundance between two samples. y ICAT is particularly useful for comparision between healthy and diseased tissue
Mass spectroscopy
their mass-to-charge (m/z) ratio. y used for determining masses of particles, for determining the elemental composition of a sample or molecule. y The molecule is first ionized which results in the formation of charged particles. The ions are separated according to their (m/z)ratio in an analyzer by electromagnetic fields.
identification
Database of proteomics
y PRIDE-The PRIDE PRoteomics IDEntifications
database is a centralised, standards compliant, public data repository for proteomics data. y PROTICdb access- PROTICdb is a tool developed by INRA that we have chosen for storing and processing our proteomic data. y The PPDB stores experimental data from in-house proteome and mass spectrometry analysis, curated information about protein function, protein properties and subcellular localization.
Organization (HUPO). y Designed to map the entire human protein set. y the three working pillars for HPP: mass spectrometry, antibodycapture,and bioinformatics tools & knowledge bases.
Application of Proteomics
y Nutrition Research y diabetes research. y Cardiovascular research. y Autoantibody profiling for the study and treatment of y y y y y y
autoimmune disease. Urological Cancer Research Fetal and maternal medicine Neurology Renal disease diagnosis. Study of Tumor Metastasis. Protein Biomarker
Description
y Predicts native-like structures for y y y y y y y
Generation Persistence Length Radius of Gyration Hydrophobicity ProRegIn Protein structure optimizer ProSEE - Scoring Function for Protein Structure Evaluation
small globular proteins Structure Generation from given dihedrals Filters for Globular Protein Evaluation Filters for Globular Protein Evaluation Filters for Globular Protein Evaluation Protein Regularity Index Energy minimizer for proteins Calculates intramolecular energy of a protein in component-wise break up.
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