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Notes On Flow Cytometry
Notes On Flow Cytometry
In acute Leukemia
Immunophenoptyping of AL
Indicated in all types of Leukemia that are not clearly myeloid to : 1. Make a positive diagnosis of ALL. 2. Diagnose unequivocally cases of AML particularly of M0 and M7 subtypes; Sometimes M6 and M5a.
Immunophenotyping Techniques
Immunoenzymatic techniques : applied to fixed slides. Immunoflourescent techniques : Antibodies are attached to flourochrome. Two techniques are used to detect a reaction : - Flourescent micrscope. - Flow cytometry.
Flow cytometry
Is a technique by which a stream of cells that have been labelled with an antibody conjugated to a flourescent dye flow past a detector and can be counted and sized. It is a rapid highly accurate and can detect several antigens on the same cells simultaneously and the strength of Ag expression.
What is Gating?
It is simply to select a single group of cells , e.g. by CD45 and SSC, and restrict further immuno-phenotyping to this group of selected cells.
M.G
Blasts
- Non-lineage related : Tdt. Second Panel : B- lymphoid : C ; SmIg. T-Lymphoid : CD1, CD5, CD4, CD 8. -If myeloid : CD14 (M4-5), anti-glycophorinA (M6), CD41 (M7).