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Molecular Biology 1-4: Put Together By: Linda Fahlberg-Stojanovska
Molecular Biology 1-4: Put Together By: Linda Fahlberg-Stojanovska
Contents
Small Molecules and Polymers
Chemical evolution
simple molecules containing C, H, O and N react to form reduced carbon-containing molecules, which then react to form organic compounds.
The process is triggered by an energy source such as sunlight or the heat released in a volcanic eruption.
simple molecules
organic compounds
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cannot find source
Small Molecules
Small Molecules: small molecular weight ,not a polymer
Examples: Metabolites and most drugs
Keywords
Membrane lipids are lipids in the cell membrane. Examples are: phospholipids, glycolipids, and cholesterol.
Alkaloids are a group of naturally occurring chemical compounds that contain mostly basic nitrogen atoms.
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http://publications.nigms.nih.gov/insidethecell/chapter1.html
http://www.bio.miami.edu/~cmallery/150/life/top_10_life.htm
Macromolecules
Macromolecules are large molecules: Proteins, Nucleic acids, Polysacharides These are polymers.
They are essential parts of organisms and participate in virtually every process within cells.
A polymer is a large molecule (macromolecule) composed of repeating structural units.
A macrocycle is a macromolecule with ring (chlorophyll).
Proteins
Proteins are composed of chains of amino acid / peptide bond Proteins are linear polymers. Many proteins are enzymes that catalyze biochemical reactions and are vital to metabolism. Proteins also have structural or mechanical functions, such as actin and myosin in muscle and the proteins in the cytoskeleton, which form a system of scaffolding that maintains cell shape. Other proteins are important in cell signaling, immune responses, cell adhesion, and the cell cycle.
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Proteins
peptide bond
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The anabolic process = condensation of two amino acids to form a peptide bond
(releases water)
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http://en.wikipedia.org/wiki/Amino_acid
Anabolic
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Polysaccharides
Polysaccharides are composed of chains of
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Polysaccharides
glycosidic bond
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Disaccharide
Nucleic Acid
Nucleic acids are composed of chains of nucleotide/ phosphodiester bond Nucleic acids include
DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic acids are linear polymers. Together with proteins, nucleic acids function in encoding, transmitting and expressing genetic information.
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Nucleic Acid
phosphodiester bond
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http://www.bio.miami.edu/dana/250/250S11_7.html
Hydrogen Bonding
The oxygen atom has a slightly negative charge. The hydrogen atoms have a slightly positive charge. This causes the molecules to line up oxygen to hydrogen with a hydrogen bond or H-bond.
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http://www.biology.arizona.edu/biochemistry/tutorials/chemistry/page3.html
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http://runningstrong-biologylibrary.blogspot.com/2010_04_01_archive.html
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http://course1.winona.edu/sberg/241f09/Lec-note/Water.htm
Chemical Polarity
Polarity underlies a number of physical properties including surface tension, solubility, melting-point and boiling-point. Polar molecules interact through dipoledipole intermolecular forces and hydrogen bonds. Molecular polarity depends on the difference in electronegativity between the atoms and the asymmetry of the molecule's structure. For example, a molecule of water is polar because of the unequal sharing of its electrons between oxygen and hydrogen in which the Oxygen has larger electronegativity than the Hydrogen, resulting in a "bent" structure. Methane is non-polar because the carbon shares the electrons with the hydrogen atoms almost uniformly.
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A hydrophobic molecule avoids water and is not water soluble. Hydrophobic molecules are non-polar. Ex: include alkanes, oils, fats
Some molecules have parts that are hydrophillic and parts that are hydrophobic Hydrophillic parts are in contact (hydrogen bond) with water, Hydrophobic parts move where the water recedes.
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http://www.exobiologie.fr/wp-content/uploads/2008/12/image1-8.jpg
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http://kvhs.nbed.nb.ca/gallant/biology/tertiary_structure.jpg
Osmosis - 1
Osmosis is the movement of solvent molecules through a semi-permeable membrane into a region of higher solute concentration, aiming to equalize the solute concentrations on the two sides. Osmosis provides the primary means by which water is transported into and out of cells. Although osmosis does not require input of energy, it does use kinetic energy and can be made to do work. Osmosis can be countered by increasing the pressure of the hypertonic solution, with respect to the hypotonic.
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Osmosis -2
Osmosis is essential in biological systems, as biological membranes are semipermeable. Semipermeable membranes are impermeable to large and polar molecules, such as ions, proteins, and polysaccharides. They are permeable to non-polar and/or hydrophobic molecules like lipids as well as to small molecules like oxygen, carbon dioxide, nitrogen, nitric oxide, etc.
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Permeability
A solvent is a liquid, solid, or gas that dissolves a solute. A solute is disolved in a solvent. Semi-permeable membrane is a membrane that is permeable to the solvent, but not the solute. semi-permeable = selectively permeable Permeability depends on solubility, charge, or chemistry, as well as solute size. Water molecules travel through the plasma membrane, vacuole or protoplast by diffusing across the phospholipid bilayer via aquaporins
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Osmotic pressure -1
Osmotic pressure is the pressure which needs to be
applied to a solution to prevent the inward flow of water across a semipermeable membrane. Osmotic pressure is a colligative property, meaning that the osmotic pressure depends on the molar concentration of the solute but not on its identity. Example: osmotic pressure of ocean water is 27 atm. Diffusion is the movement of particles from a region of higher concentration to one of lower concentration (this is freeflow there is no membrane). Solutes able to freely cross the membrane do not exert osmotic pressure because they will always be in equal concentrations on both sides of the membrane. 34
Osmotic pressure -2
Example: Salt water is lighter so has greater osmotic pressure than pure water.
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http://www.sparknotes.com/chemistry/solutions/colligative/section1.html
Tonicity - 1
Tonicity measures the difference in the osmotic pressures. When the osmotic pressure of the solution outside a cell is higher than the osmotic pressure inside the blood cells, the solution is hypertonic. Example: Normal salt content of a red blood cell is 9g/L
HypERtonic: salt content outside the RBC is >9g/L (The osmotic pressure is greater outside, water will EXIT the RBC and cause shriveling.) Isotonic: salt content outside the RBC is 9g/L. (The osmotic pressure is equal on both sides.) HypOtonic: salt content outside the RBC is <9g/L (The osmotic pressure is smaller outside, water will ENTER the RBC and cause swelling.)
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Tonicity - 2
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Water travels from the region of low concentration of solute to the region of high concentration of solute in order to equalize the concentrations .
water
hypotonic environment
hypertonic environment
Water molecules travel through the plasma membrane, vacuole or protoplast by diffusing across the phospholipid bilayer via aquaporins.
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Water self-ionization - 1
The self-ionization of water is the reversible chemical reaction in which a proton is transferred from one water molecule to another, in pure water or an aqueous solution to create the two ions: hydronium H3O+ and hydroxide OH.
The self-ionization of water depends on temperature and pressure.
In autoprotolysis is the transfer of a proton between two identical molecules. Every solvent containing hydrogen can undergo autoprotolysis.
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Water self-ionization - 2
The equilibrium constant of water is:
At 25 C, Kw 1.01014.
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pH
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http://packerpedia.wiki.packer.edu/file/view/pH_Scale.jpg
pH
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http://packerpedia.wiki.packer.edu/file/view/pH_Scale.jpg
Enzymes
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. Names of Enzymes - often end in ASE
hydrolase (digestive enzyme catalyzes hydrolysis) toplomerase (regulation of unwinding of DNA)
Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. A substrate is a reactant that has been acted upon by a catalyst. In enzymatic reactions, the reactants are called substrates.
Enzymes
In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products.
http://en.wikipedia.org/wiki/Enzyme#Cofactors_and_coenzymes
http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20101/bio%20101%20lectures/energy/energy.htm
Enzymes
Enzymes are selective for their substrates. So, the set of enzymes made in a cell determines which metabolic pathways occur in that cell. Like all catalysts, enzymes work by lowering the activation energy (Ea) for a reaction, thus dramatically increasing the rate of the reaction.
Enzyme Classification
EC 1 Oxidoreductases: catalyze oxidation/reduction reactions EC 2 Transferases: transfer a functional group (e.g. a methyl or phosphate group) EC 3 Hydrolases: catalyze the hydrolysis of various bonds EC 4 Lyases: cleave various bonds by means other than hydrolysis and oxidation EC 5 Isomerases: catalyze isomerization changes within a single molecule EC 6 Ligases: join two molecules with covalent bonds.
Cofactors
Some enzymes do not need any additional components to show full activity. However, others require non-protein molecules called cofactors to be bound in order to activate. Cofactors can be either inorganic (e.g., metal ions and iron-sulfur clusters) or organic compounds (e.g., flavin and heme). Organic cofactors can be either
prosthetic groups (tightly bound to an enzyme) or coenzymes (released from the enzyme's active site during the reaction).
Cofactors
Cofactors
Coenzymes
Coenzymes include NADH, NADPH and ATP adenosine triphosphate. These molecules transfer chemical groups between enzymes.
Enzyme Inhibitors
MichaelisMenten Equation
In biochemistry, MichaelisMenten kinetics is one of the simplest and best-known models of enzyme kinetics. In biology, kinetics is the rate of reactions. MichaelisMenten kinetics describes the rate of enzymatic reactions, by relating concentration [S] of a substrate S reaction rate v MichaelisMenten Equation constant Km
Here, notice that the fraction has NO unit (everything is concentrations). On the next page, notice that Km depends on both the curve and Vmax . So Km is found by experiment (empirically) and given in tables.
MichaelisMenten Constant
If Vmax represents the maximum reaction velocity achieved at saturating substrate concentrations
Then, the Michaelis constant Km is the substrate concentration at which the reaction rate is half of Vmax Biochemical reactions involving a single substrate are often assumed to follow MichaelisMenten kinetics.