Scribd Logo
Upload

Welcome to Scribd!

  • Cell-Based Autonomous Biosensing Microsystem: Livesense

    Uploaded by

    nanoteraCH
    138 views20 pages
    The project aims to develop an autonomous cell-based biosensor microsystem using various cell models for environmental monitoring applications. Several research groups will work on integrating cell culture chambers, sensors to measure cell response, and processing controls. The goal is to validate the system's ability to detect toxic substances and potentially deploy it for remote environmental sampling.

    Original Description:

    Original Title

    1204_Livesense

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    PPT, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The project aims to develop an autonomous cell-based biosensor microsystem using various cell models for environmental monitoring applications. Several research groups will work on integrating cell culture chambers, sensors to measure cell response, and processing controls. The goal is to validate the system's ability to detect toxic substances and potentially deploy it for remote environmental sampling.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    138 views20 pages

    Cell-Based Autonomous Biosensing Microsystem: Livesense

    Uploaded by

    nanoteraCH
    The project aims to develop an autonomous cell-based biosensor microsystem using various cell models for environmental monitoring applications. Several research groups will work on integrating cell culture chambers, sensors to measure cell response, and processing controls. The goal is to validate the system's ability to detect toxic substances and potentially deploy it for remote environmental sampling.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    of 20

    Cell-based autonomous biosensing microsystem

    LiveSense

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    Scope of the project


    Demonstrate an autonomous cell-based biosensor microsystem for environmental remote monitoring applications

    Scientific objectives: Study various cell models for toxicology assays in microbioreactor format Develop new physical methods for measuring cell response in situ Develop a micro-bioreactor with intergated sensors
    07/05/2012 Nano-Tera.ch Annual Plenary Meeting 2

    General concept
    TER Z(f)

    >
    >
    Nutrients + Temp. control

    < fluo v F

    Data comm

    osm

    Signal processing control glu pH I

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    Cell models:

    Bacteria in beads, Unil

    Hepatocytes on-chip, EPFL_LMIS

    Epithelial cells on chip, CSEM

    Fibroblats on nanopillars, ETHZ

    07/05/2012

    nano-tera.ch annual meeting

    The 8 teams in LiveSense project


    EPFL-LMIS Microsystems Laboratory Microfluidics, cell chips, bio-impedance UNIL-DMF Department of Fundmental Biology Cell biology, gene reporters, bacterial sensors Philippe Renaud Jan van der Meer Martial Geiser Viola Vogel Martha Liley Hubert Girault Nico de Rooij Peter van der Val Michael Riediker

    HESSO-ISI Industrial Systems Institute Microsystems, electronics, optical sensors ETHZ-MAT Biologically Oriented Materials Biomaterial, cell biology CSEM Nanobiotechnology group Surface biochemistry, biomaterials

    EPFL-LEPA Electrochemistry and Analytics Laboratory Electrochemical sensing, analytical chemistry EPFL-IMT UNIL-IST Sensors and Actuators Laboratory Microfluidics, microsensors Institute of Occupational Health Health effect of pollution

    07/05/2012

    nano-tera.ch annual meeting

    Bacterial biosensor: fluorescence detection


    TER Z(f)

    >
    >
    Nutrients + Temp. control Nutrients

    < fluo fluo v F

    Data comm

    osm

    Signal processing control glu pH I

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    Livesense partner UNIL, Lausanne

    Bacterial biosensor: amperometric detection


    TER Z(f)

    >
    >
    Nutrients + Temp. control Nutrients

    < fluo v v F

    Data comm

    osm

    Signal processing control glu pH II

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    Livesense partner EPFL-LEPA, Lausanne

    Epithelial cell biosensor: TER measurement


    TER TER Z(f)

    >
    >
    Nutrients + Temp. control

    < fluo v F

    Data comm

    osm

    Signal processing control glu pH I

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    10

    Livesense partner CSEM, Neuchtel

    Epithelial cell biosensor: glucose, pH, osmo


    TER Z(f)

    >
    >
    Nutrients + Temp. control

    < fluo v v F

    Data comm

    osm

    Signal processing control glu glu pH pH I

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    12

    Livesense partner EPFL-IMT, Neuchtel

    Cell-based toxicology: force measurement


    TER Z(f)

    >
    >
    Nutrients + Temp. control

    < fluo v Z(f) F

    Data comm

    osm

    Signal processing control glu pH I

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    14

    Li

    Livesense partner ETHZ, Zrich

    Cell-based toxicology: impedance spectroscopy


    TER Z(f)

    >
    >
    Nutrients + Temp. control

    < fluo v F

    Data comm

    osm

    Signal processing control glu pH I

    07/05/2012

    Nano-Tera.ch

    Annual Plenary Meeting

    16

    Livesense partner EPFL, Lausanne

    System integration:

    Microfluidic cell incubator Fluorescence sensors processor + GSM module Power supply

    Micropumps for perfusion

    07/05/2012

    nano-tera.ch annual meeting

    18

    Livesense partner HEVS, Sion

    Summary
    A set of cell models, cultivable in microenvironments Several readout schemes for monitoring cell response System integration for bacteria and epithelial cell New techniques for toxicology screening

    Next steps:
    Validation with toxicants Environmental sampling

    07/05/2012

    nano-tera.ch annual meeting

    20

    You might also like