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    The differential count of WBC identifies the percentage of different types of white blood cells in a stained blood film. The main white blood cell types identified are neutrophils, which have a multi-lobed nucleus and make up 50-70% of WBCs; lymphocytes, which have a rounded nucleus and comprise 20-40%; monocytes, with a large kidney-shaped nucleus accounting for 2-8%; eosinophils with a two-lobed nucleus; and basophils, which have an overlapped nucleus, representing 0-1% of WBCs. The differential count provides information about the relative proportions of white blood cells.

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    How to identify different type of WBC in Microscope.

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    The differential count of WBC identifies the percentage of different types of white blood cells in a stained blood film. The main white blood cell types identified are neutrophils, which have a multi-lobed nucleus and make up 50-70% of WBCs; lymphocytes, which have a rounded nucleus and comprise 20-40%; monocytes, with a large kidney-shaped nucleus accounting for 2-8%; eosinophils with a two-lobed nucleus; and basophils, which have an overlapped nucleus, representing 0-1% of WBCs. The differential count provides information about the relative proportions of white blood cells.

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    274 views10 pages

    Differential Count of WBC

    Uploaded by

    Bangla Car
    The differential count of WBC identifies the percentage of different types of white blood cells in a stained blood film. The main white blood cell types identified are neutrophils, which have a multi-lobed nucleus and make up 50-70% of WBCs; lymphocytes, which have a rounded nucleus and comprise 20-40%; monocytes, with a large kidney-shaped nucleus accounting for 2-8%; eosinophils with a two-lobed nucleus; and basophils, which have an overlapped nucleus, representing 0-1% of WBCs. The differential count provides information about the relative proportions of white blood cells.

    Copyright:

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    Differential Count of WBC

    What is the differential Count of WBC?


    Definition: The counting of different types of white blood cells in a stained blood film to find out their percentage is called Differential Count of WBC.

    Identification of WBC

    Neutrophils
    Features of Neutrophils:

    Nucleus of neutrophils contains multiple lobes (3-5), connected by chromatin filaments Nuclear stain: Violet 50-70%

    Lymphocytes
    Features of Lymphocytes Nucleus: Rounded ( So no lobed) 20-40%

    Monocyte

    Features of Monocyte Nucleus: Kidney Shaped, Large 2-8%

    Eosinophils
    Features of Eosinophils: Nucleus: Bilobed (2-lobed)

    Basophils
    Features of Basophil Nucleus: Usually overlapped with granules Percentage: 0-1%

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